Results 21 to 30 of about 12,002 (336)

Investigation of enhanced intracellular delivery of nanomaterials modified with novel cell-penetrating zwitterionic peptide-lipid derivatives

Drug Delivery, 2023
Functionalized drug delivery systems have been investigated to improve the targetability and intracellular translocation of therapeutic drugs. We developed high functionality and quality lipids that met unique requirements, focusing on the quality of ...
Yuri Sugimoto, Tadaharu Suga, Mizuki Umino, Asako Yamayoshi, Hidefumi Mukai, Shigeru Kawakami   +5 more
doaj   +1 more source

Surface Plasmon Resonance Based Binding Characterization for Screening RNA-Loaded Lipid Nanoparticles (LNPs): Exploring Species Cross-Reactivity in LNP-Apolipoprotein E Interactions. [PDF]

Mol Pharm
Lew B, Chhabra S, Lewis JA, Wagoner AC, Hsu A, Halaby S, Sharma P, Murray JK, Kinderman F, Piedmonte DM, Amer BR.   +10 more
europepmc   +3 more sources

Importance of Process Parameters Influencing the Mean Diameters of siRNA-Containing Lipid Nanoparticles (LNPs) on the in Vitro Activity of Prepared LNPs.

Biological and Pharmaceutical Bulletin, 2022
Genetic drugs have the potential to treat a variety of diseases. Recently, lipid nanoparticles (LNPs) have attracted much attention among drug delivery systems for genetic drugs.
Kazuya Nakamura, K. Aihara, T. Ishida
semanticscholar   +1 more source

Messenger RNA in lipid nanoparticles rescues HEK 293 cells from lipid-induced mitochondrial dysfunction as studied by real time pulse chase NMR, RTPC-NMR, spectroscopy

Scientific Reports, 2022
Analytical tools to study cell physiology are critical for optimizing drug-host interactions. Real time pulse chase NMR spectroscopy, RTPC-NMR, was introduced to monitor the kinetics of metabolite production in HEK 293T cells treated with COVID-19 ...
Nicholas Sciolino, Sergey Reverdatto, Aaron Premo, Leonard Breindel, Jianchao Yu, Gregory Theophall, David S. Burz, Anna Liu, Todd Sulchek, Ann Marie Schmidt, Ravichandran Ramasamy, Alexander Shekhtman   +11 more
doaj   +1 more source

Validation of an HPLC-CAD Method for Determination of Lipid Content in LNP-Encapsulated COVID-19 mRNA Vaccines

Vaccines, 2023
Lipid nanoparticles (LNPs) are widely used as delivery systems for mRNA vaccines. The stability and bilayer fluidity of LNPs are determined by the properties and contents of the various lipids used in the formulation system, and the delivery efficiency ...
Xiaojuan Yu, Chuanfei Yu, Xiaohong Wu, Yu Cui, Xiaoda Liu, Yan Jin, Yuhua Li, Lan Wang   +7 more
doaj   +1 more source

High-Resolution Characterization of Protein-Conjugated, mRNA-Loaded Lipid Nanoparticles by Analytical Ultracentrifugation. [PDF]

Adv Funct Mater
Lipid nanoparticles containing messenger RNA are characterized by sedimentation velocity analytical ultracentrifugation using UltraScan's new Custom Grid algorithm to provide multi‐dimensional distributions for partial specific volume (particle density), molar mass, sedimentation, diffusion, and hydrodynamic radius.
Bird S, Smith C, Habibi N, Rivera S, Mortezazadeh S, Martin R, Geilich BM, Besin G, Demeler B.   +8 more
europepmc   +2 more sources

Effect of PEG Anchor and Serum on Lipid Nanoparticles: Development of a Nanoparticles Tracking Method

Pharmaceutics, 2023
Polyethylene glycol (PEG) is used in Lipid Nanoparticles (LNPs) formulations to confer stealth properties and is traditionally anchored in membranes by a lipid moiety whose length significantly impacts the LNPs fate in vivo.
Manon Berger, Manon Degey, Jeanne Leblond Chain, Erik Maquoi, Brigitte Evrard, Anna Lechanteur, Géraldine Piel   +6 more
doaj   +1 more source

Payload distribution and capacity of mRNA lipid nanoparticles

Nature Communications, 2022
Lipid nanoparticles (LNPs) are effective vehicles to deliver mRNA vaccines and therapeutics but assessing the mRNA packaging characteristics in LNPs is challenging.
Sixuan Li, Yizong Hu, Andrew Li, Jinghan Lin, Kuangwen Hsieh, Zachary Schneiderman, Pengfei Zhang, Yining Zhu, Chenhu Qiu, Efrosini Kokkoli, Tza-Huei Wang, Hai-Quan Mao   +11 more
doaj   +1 more source

Elucidation of lipid nanoparticle surface structure in mRNA vaccines

Scientific Reports, 2023
Lipid nanoparticles (LNPs) have been used as a carrier for messenger RNA (mRNA) vaccines. Surface properties of LNPs are important to the stability and function of mRNA vaccines. Polyethylene-glycol (PEG) is a functional lipid at the surface of LNPs that
Mingzhang Maple Wang, Caitlin N. Wappelhorst, Erika L. Jensen, Ying-Chih Thomas Chi, Jason C. Rouse, Qin Zou   +5 more
doaj   +1 more source

Multicomponent Lipid Nanoparticles for RNA Transfection

Pharmaceutics, 2023
Despite the wide variety of available cationic lipid platforms for the delivery of nucleic acids into cells, the optimization of their composition has not lost its relevance.
Nataliya Gretskaya, Mikhail Akimov, Dmitry Andreev, Anton Zalygin, Ekaterina Belitskaya, Galina Zinchenko, Elena Fomina-Ageeva, Ilya Mikhalyov, Elena Vodovozova, Vladimir Bezuglov   +9 more
doaj   +1 more source