Results 271 to 280 of about 381,633 (319)
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Assay of an activator for lipoprotein lipase
Clinica Chimica Acta, 1976A method is described which enables the ability of human serum to activate guinea pig lipoprotein lipase to be measured in terms of an arbitrary standard. Evidence is offered which strongly suggests that the substance measured is also the activator of human lipoprotein lipase and observations on the relative levels of activator in human sera are given.
Peter Chu, A.L. Miller, G.L. Mills
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Postheparin Lipoprotein Lipases
New England Journal of Medicine, 1975A heterogeneous group of enzymes, including hepatic and extrahepatic lipases, monoglyceride hydrolase, and phospholipase, are released into plasma after heparin injection, and may function in clear...
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Atherosclerosis, 1975
Rat heart lipoprotein lipase was highly purified by affinity chromatography using heparin-Sepharose 4B. When extracts of acetone powder were applied to columns, lipase activity was firmly bound to the gel matrix and was later eluted with 1.5 M NaCl. At this stage the eluted enzyme was purified 1500-fold.
Jer-Shung Twu +5 more
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Rat heart lipoprotein lipase was highly purified by affinity chromatography using heparin-Sepharose 4B. When extracts of acetone powder were applied to columns, lipase activity was firmly bound to the gel matrix and was later eluted with 1.5 M NaCl. At this stage the eluted enzyme was purified 1500-fold.
Jer-Shung Twu +5 more
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Interaction of Lipoprotein Lipase with Heparina
Annals of the New York Academy of Sciences, 1989The hydrolysis of triglyceride-rich plasma lipoproteins is initiated by lipoprotein lipase (LPL) located at the luminal surface of endothelial cells. We previously reported that LPL binds to cultured endothelial cells with a Km of 2.7 x 10(-7) M and that this binding is inhibited by heparinase, heparin, or heparan sulfate.
Kazuhiro Oka +3 more
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Lipoprotein lipase in the physiological system
Biochemical Society Transactions, 1985Lipoprotein lipase (EC 3.1.1.34) cataly:%es the hydrolysis of plasma triacylglycerols and thereby enables the transfer of triacylglycerol fatty acids from the circulation to various extrahepatic tissues. The enzyme is synthesized in the parenchymal cells of a number of tissues including adipose tissue, heart and mammary gland, and is then secreted and ...
Donald S. Robinson +2 more
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Lipoprotein lipase: from gene to obesity.
American Journal of Physiology. Endocrinology and Metabolism, 2009Lipoprotein lipase (LPL) is a multifunctional enzyme produced by many tissues, including adipose tissue, cardiac and skeletal muscle, islets, and macrophages. LPL is the rate-limiting enzyme for the hydrolysis of the triglyceride (TG) core of circulating
Hong Wang, R. Eckel
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1993
Lipoprotein lipase (LPL) activity in cultured mesenchymal cells derived from new-born rat heart, increases during the final week after plating and then plateaus [1, 2]. Expression of LPL requires the presence of 20% serum and activity decreases markedly in serum-poor medium [3,4], Basic fibroblast growth factor (bFGF) plays an important role in cell ...
Gideon Friedman +2 more
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Lipoprotein lipase (LPL) activity in cultured mesenchymal cells derived from new-born rat heart, increases during the final week after plating and then plateaus [1, 2]. Expression of LPL requires the presence of 20% serum and activity decreases markedly in serum-poor medium [3,4], Basic fibroblast growth factor (bFGF) plays an important role in cell ...
Gideon Friedman +2 more
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Binding of deoxycholate to lipoprotein lipase
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1979Abstract Binding of deoxycholate to lipoprotein lipase from bovine milk was demonstrated by equilibrium dialysis and by charge shift electrophoresis. The detergent increased the solubility and stability of the enzyme in aqueous buffers, but did not change its elution position on gel filtration.
Gunilla Bengtsson, Thomas Olivecrona
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A further characterization of lipoprotein lipase
Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism, 1968Abstract The isolation of hydrolysis products from glyceryl [1- 14 C]trioleate provided a sensitive and reproducible assay system to measure plasma post-heparin lipolytic activity and adipose tissue lipoprotein lipase (glycerol-ester hydrolase, EC 3.1.1.3). Essentially no lipolytic activity was detected in pre-heparin plasma.
Heiner Greten +2 more
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Mechanism of action of lipoprotein lipase
1986Publisher Summary This chapter describes several of the methods used to determine the mechanism of action of lipoprotein lipase purified from bovine milk and the effect of apoC-II on lipoprotein lipase (LpL) catalysis. LpL is located primarily in adipose tissue, lung, muscle, and lactating mammary gland.
Lilian Socorro +4 more
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