Results 71 to 80 of about 6,682,001 (239)

Importin 7 mediates the nuclear import of HIV‐1 integrase via a specific interacting interface

FEBS Open Bio, EarlyView.
HIV‐1 integrase enables viral DNA integration into the host genome. By binding to the core domain of the host protein Importin 7 via its C‐terminal domain, the integrase is transported across the nuclear membrane into the nucleus, where integration of the viral genome into host DNA takes place. This translocation is a critical step for subsequent viral
Juana Bana, Avigail Yariv, Tal Oppenheim, Hadar Amartely, Dana Reichmann, Oded Livnah   +5 more
wiley   +1 more source

High-yield production of scutellaria radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis root-derived cells

Brazilian Archives of Biology and Technology, 2009
Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 ...
Takashi Ohtsuki, Mika Himeji, Harumi Fukazawa, Miho Tanaka, Hisako Yamamoto, Akio Mimura   +5 more
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

FEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas, Aaron D. Morgan, Ignas Čiurlionis, Christian Böhm, Stefan Jockenhövel, Willi Jahnen‐Dechent, Marc A. M. J. van Zandvoort   +6 more
wiley   +1 more source

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Comparison of Mycobacterium tuberculosis drug susceptibility using solid and liquid culture in Nigeria. [PDF]

, 2013
BACKGROUND: This study compares Mycobacterium tuberculosis culture isolation and drug sensitivity testing (DST) using solid (LJ) and liquid (BACTEC-MGIT-960) media in Nigeria.
Luis E Cuevas, Abdurrahman, Saddiq T, Yassin, Mohammed A, Sogaolu, Olumide M., Saddiq T Abdurrahman, Juliana O Lawson, Yassin, Mohammed A., Uzoewulu, Gertrude N, Juliana N Ebisike, Nanmdi Emenyonu, Lawson, Lovett, Mohammed A Yassin, Abdurrahman, Saddiq T., Uzoewulu, Gertrude N., Parry, Christopher M, Gertrude N Uzoewulu, Lawson, Juliana O., Lawson, Juliana O, Cuevas, Luis E, Ebisike, Juliana N., Christopher M Parry, Emenyonu, Nanmdi, Parry, Christopher, Olumide M Sogaolu, Sogaolu, Olumide M, Ebisike, Juliana N, Lovett Lawson, Cuevas, Luis   +27 more
core   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Evaluation of Quantity and Hyperhydricity of Cocoa Somatic Embryo Obtained from Solid Culture, Liquid Culture, and Sequence Subculture

Coffee and Cocoa Research Journal, 2013
Research  aimed  to  study  the  effect  of  solid  culture,  liquid  culture,  and sequence  subculture  on  quantity  and  hyperhydricity  of  somatic  embryo  was carried  out  at  Laboratory  of  Biotechnology,  Indonesian  Coffee  and  Cocoa ...
Sulistyani Pancaningtyas
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Special Commemorative Issue

, 1975
Newspaper publication from Liquid Paper Corporation on Letter Perfect, November 1975, with updates to staff and patrons about the on-goings and news updates regarding the ...
Liquid Paper Corporation
core  

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source