Results 211 to 220 of about 702,225 (266)

Growing Bacteriophage M13 in Liquid Culture

Cold Spring Harbor Protocols, 2006
Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Joseph, Sambrook, David W, Russell
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Liquid Culture Production of Fungal Microsclerotia

2016
Fungal microsclerotia ("small" sclerotia) are compact hyphal aggregates, typically 50-600 μm in diameter, that are formed under unfavorable nutritional and/or environmental conditions. These structures are often melanized and desiccated to some degree containing endogenous nutritional reserves for use when favorable conditions return.
Mark A, Jackson, Angela R, Payne
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Liquid-Overlay Culture of Cellular Spheroids

1984
The capacity of different types of human cells to form and grow as spheroids was tested by using suspension culture over a bottom surface, which prevented cell attachment (liquid-overlay culture). The best and most convenient bottom surface so far tested was the bottom of a normal culture dish covered with a thin film of agarose.
J, Carlsson, J M, Yuhas
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Cultural dispersion and stock liquidity

Global Strategy Journal, 2023
AbstractResearch SummaryThis paper examines whether cultural dispersion of a multinational firm affects its stock trading in the market. The result shows that the stock liquidity negatively relates to the degree of cultural dispersion, suggesting it is costlier to trade stocks of culturally diverse firms. Among five measures of stock liquidity, Chae's (
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Culture of Trichophyton verrucosum in a liquid medium

Mycoses, 2009
Summary The possibility of application of Sabourauds liquid medium for isolation and multiplication of Trichophyton verrucosum was investigated. It was found that the use of the liquid medium for isolation of the fungus from the infected material produced the growth of T. verrucosum within 9–11 days; the medium did not get dry during the cultivation
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A SHAKING DEVICE FOR LIQUID CULTURES

Journal of Applied Bacteriology, 1957
SUMMARY: Details are given for the construction of a reciprocating shaking device for liquid cultures. Up to 36 litres can be shaken at one time. The machine is compact, the base being only 28 in. square.
A. P. MULCOCK, B. J. ABLEY
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THE LIQUID MEDIUM IN TISSUE CULTURE

Biological Reviews, 1954
SummaryIn the past, largely on an empirical basis, it was found that the most effective liquid media for culturing animal tissue cells consisted of mixtures of physiological saline solutions, tissue extracts (preferably from embryonic tissue) and serum or plasma.
D. C. STEWART, PAUL L. KIRK
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Enhanced retroviral transduction of 293 cells cultured on liquid-liquid interfaces

Biotechnology and Bioengineering, 2000
In clinical research, retrovirus-mediated gene therapy is one of the most commonly used methods to deliver and express the gene of interest due its ability to allow for stable gene integration into the chromosomes of target cells. To elevate the efficiency of viral transduction, several restrictions, such as low virus-cell encounters and the necessity ...
Y J, Kwon, H, Yu, C A, Peng
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Microbial transformation and sorption of anthracene in liquid culture

Bioprocess and Biosystems Engineering, 2012
Armillaria sp. F022, a white-rot fungus isolated from decayed wood in tropical rain forest was used to biodegrade anthracene in cultured medium. The percentage of anthracene removal by Armillaria sp. F022 reached 13 % after 7 days and at the end of the experiment, anthracene removal level was at 87 %.
Hadibarata, T., Zubir, M. M., Rubiyatno, Rubiyatno, Chuang, T. Z.   +3 more
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Liquid nitrogen storage of cultured T lymphocytes

Journal of Immunological Methods, 1985
The conditions required for storing and recovering IL-2-dependent T cell clones from liquid nitrogen were investigated. For maximum cell recovery, cultured T lymphocytes were precooled at 4 degrees C for 15 min in medium containing 10% DMSO and 20% FCS before storage in liquid nitrogen.
P L, Mottram, J L, McKimm-Breschkin, J F, Miller   +2 more
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