Results 111 to 120 of about 4,230,776 (271)
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Sound Stimulation Can Affect Saccharomyces cerevisiae Growth and Production of Volatile Metabolites in Liquid Medium. [PDF]
Metabolites, 2021 Harris A +4 more
europepmc +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
The Influence of Liquid Medium Choice in Determination of Minimum Inhibitory Concentration of Essential Oils against Pathogenic Bacteria. [PDF]
Antibiotics (Basel), 2022 Hulankova R.
europepmc +1 more source
Comparative assessment of crystallographic and cryo‐EM models in the Protein Data Bank
FEBS Open Bio, EarlyView.Raw data obtained by X‐ray crystallography or cryo‐EM result in experimental maps, ultimately fitted by atomic models. Although the physical principles are different, the final results can be viewed, compared, and evaluated in the same way. With cryogenic electron microscopy (cryo‐EM) on track to surpass X‐ray crystallography as the preferred method ...
Alexander Wlodawer +7 more
wiley +1 more source
FEBS Open Bio, EarlyView.In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey +4 more
wiley +1 more source
Influence of Laser Process Parameters, Liquid Medium, and External Field on the Synthesis of Colloidal Metal Nanoparticles Using Pulsed Laser Ablation in Liquid: A Review. [PDF]
Nanomaterials (Basel), 2022 Subhan A, Mourad AI, Al-Douri Y.
europepmc +1 more source
FEBS Open Bio, EarlyView.Human ABCE1 cannot functionally replace its yeast ortholog. Yeast–human chimera analysis identified NBD1 as a major interspecies barrier. Genetic screening yielded hABCE1 revertants that rescue yeast viability but fail to suppress aberrant translation reinitiation in the 3′ UTR.
Eriko Nakata +3 more
wiley +1 more source
Механика машин, механизмов и материалов, 2010 The present paper is devoted to investigation of vibrations of orthotropic cylindrical shells filled by flowing liquid in infinitely elastic medium.
F.S. Latifov , F.A. Seifullaev
doaj
, 2012 The flow dynamics of a unidirectional, spontaneous capillary flow of a wetting liquid into porous medium is investigated numerically and experimentally.
Zand, Ali R. +9 more
core +1 more source