Results 121 to 130 of about 6,900,102 (340)

YlmG1 is localized exclusively to the chloroplast envelope membrane and is involved in preprotein translocation in Arabidopsis thaliana

open access: yesFEBS Open Bio, EarlyView.
Cytosolically synthesized chloroplast preproteins are translocated across the outer and inner envelope membranes through translocons called TOC and TIC, respectively. In green algae and plants, the TIC core is composed of essential membrane proteins, Tic12, Tic20, and Tic214.
Mengyi Li, Xueyang Zhao, Masato Nakai
wiley   +1 more source

Optimizing Micropropagation of Tanacetum balsamita L.: A Machine Learning Approach to Compare Semisolid Media and Temporary Immersion System

open access: yesHorticulturae
Tanacetum balsamita L. is a medicinal and aromatic plant of high economic value, yet its tissue culture and micropropagation protocols remain poorly developed.
Carla Benelli   +4 more
doaj   +1 more source

Computer-aided high-throughput cloning of bacteria in liquid medium

open access: yesBioTechniques, 2011
Bacterial cloning was first introduced over a century ago and has since become one of the most useful procedures in biological research, perhaps paralleled in its ubiquity only by PCR and DNA sequencing.
Tuval Ben Yehezkel   +6 more
doaj   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

open access: yesFEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková   +3 more
wiley   +1 more source

Liquid medium culture: an approach for the commercial micropropagation of aloe (Aloe barbadensis Mill.)

open access: yesRevista Colombiana de Biotecnología, 2015
Micropropagation is considered a successful alternative for aloe (Aloe barbadensis Mill.) plant production. However, it has limited use due to the high production cost.
Nilca Rosa Albany   +5 more
doaj   +1 more source

A Method to Directly Identify Cronobacter sakazakii in Liquid Medium by MALDI-TOF MS. [PDF]

open access: yesFoods, 2023
Song D   +8 more
europepmc   +1 more source

Draining of Liquid from a Well into a Porous Medium [PDF]

open access: yesThe Quarterly Journal of Mechanics and Applied Mathematics, 2000
Summary: Capillary suction and gravity force liquid from a well into surrounding porous medium. The position of the wet/dry boundary is determined, together with the time-dependent level of the liquid in the well. Two models for the structure of the porous medium are employed. In the first, a one-dimensional model, the medium is formed from an assembly
openaire   +1 more source

Evolutionarily divergent DUF4465 domains have a common vitamin B12‐binding function

open access: yesFEBS Open Bio, EarlyView.
We show that DUF4465 family proteins, widespread across bacteria from gut microbiomes, hydrothermal vents, and soil, share a common vitamin B12‐binding function. These augmented β‐jellyroll proteins bind vitamin B12 via extended loops. Our findings establish sequence‐diverse DUF4465 proteins as a widespread class of B12‐binding proteins, highlighting ...
Charlea Clarke   +4 more
wiley   +1 more source

Large‐scale bidirectional arrayed genetic screens identify OXR1 and EMC4 as modifiers of αSynuclein aggregation

open access: yesFEBS Open Bio, EarlyView.
Activation of the mitochondrial protein OXR1 increases pSyn129 αSynuclein aggregation by lowering ATP levels and altering mitochondrial membrane potential, particularly in response to MSA‐derived fibrils. In contrast, ablation of the ER protein EMC4 enhances autophagic flux and lysosomal clearance, broadly reducing α‐synuclein aggregates.
Sandesh Neupane   +11 more
wiley   +1 more source

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