Results 31 to 40 of about 819,719 (249)

In Vitro Propagation of Rheophytic Orchid, Epipactis flava Seidenf.—A Comparison of Semi-Solid, Continuous Immersion and Temporary Immersion Systems

Biology, 2019
Epipactis flava Seidenf. is an endangered Thai rheophytic orchid that has recently shown a rapid decrease in its natural habitat, prompting an urgent need for conservation using ex situ reintroduction methods.
Boworn Kunakhonnuruk, Phithak Inthima, Anupan Kongbangkerd   +2 more
doaj   +1 more source

Effect of Liquid Culture Systems (Temporary Immersion Bioreactor and Rotary Shaker) Used During Multiplication and Differentiation on Efficiency of Repetitive Somatic Embryogenesis of Narcissus L. ‘Carlton’

Agronomy
Liquid culture systems, including bioreactors, are valuable tools for the scaling up of production. Their involvement leads to the automation of the highly efficient, reproducible somatic embryogenesis of Narcissus L. ‘Carlton’.
Małgorzata Malik, Ewelina Tomiak, Bożena Pawłowska   +2 more
doaj   +1 more source

In vitro degradation of diacetoxyscirpenol and T-2 toxin by use of Mucor racemosus fresen. f. racemosus isolate [PDF]

Zbornik Matice Srpske za Prirodne Nauke, 2011
Under controlled in vitro conditions the capacity of the Mucor racemosus f. racemosus 1215/09 isolate to degrade type A trichothecenes (diacetoxyscirpenol - DAS and T-2 toxin) was observed in the liquid nutritive medium.
Bočarov-Stančić Aleksandra S., Stanković Slavica Ž., Lević Jelena T., Salma Nataša M., Pantić Vladimir R., Barnić Saša S.   +5 more
doaj   +1 more source

Effect of liquid media culture systems on yam plant growth (Dioscorea alata L. 'Pacala Duclos') [PDF]

Biotechnologie, Agronomie, Société et Environnement, 2011
The culture system type in liquid media influences yam plant growth ('Pacala Duclos' clone). In culture systems with forced renewal of internal atmosphere in culture flasks, Temporary Immersion System (TIS) and Constant Immersion System (CIS) with ...
Jova, MC., Kosky, RG., Cuellar, EE.
doaj  

Vaso fermentador de bajo costo para la micropropagación masiva de jengibre

Agronomía Mesoamericana, 2007
Vaso fermentador de bajo costo para la micropropagación masiva de jengibre. La presente investigación se realizó en el Centro de Investigación en Biotecnología del Instituto Tecnológico de Costa Rica, durante el primer semestre del 2003 y tuvo como ...
Alejandro Hernández Soto, Andrés M. Gatica-Arias, Silvana Alvarenga-Venutolo   +2 more
doaj   +1 more source

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Multiplicación in vitro de segmentos nodales del clon de ñame Blanco de Guinea (Dioscorea cayenensis - D. rotundata ) en sistemas de cultivo semiautomatizado

Revista Colombiana de Biotecnología, 2009
Con el empleo del sistema de inmersión temporal fue posible incrementar la multiplicación in vitro de los segmentos nodales en el clon de ñame Blanco de Guinea.
Manuel Cabrera Jova, Rafael Gómez Kosky, Sergio Rodríguez Morales, Jorge López Torres, Aymé Rayas Cabrera, Milagros Basail Pérez, Arletys Santos Pino, Víctor Medero Vega, Germán Rodríguez Rodríguez   +8 more
doaj   +2 more sources

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source