Results 41 to 50 of about 3,984,547 (275)
Calpain small subunit homodimerization is robust and calcium‐independent
FEBS Letters, EarlyView.Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy +4 more
wiley +1 more source
Liquid - liquid flows in microchannels [PDF]
, 2011 This paper was presented at the 3rd Micro and Nano Flows Conference (MNF2011), which was held at the Makedonia Palace Hotel, Thessaloniki in Greece.
Dore, V +3 more
core +6 more sources
Revista Colombiana de Biotecnología, 2009 Con el empleo del sistema de inmersión temporal fue posible incrementar la multiplicación in vitro de los segmentos nodales en el clon de ñame Blanco de Guinea.
Manuel Cabrera Jova +8 more
doaj +2 more sources
Effect of liquid media culture systems on yam plant growth (Dioscorea alata L. 'Pacala Duclos') [PDF]
Biotechnologie, Agronomie, Société et Environnement, 2011 The culture system type in liquid media influences yam plant growth ('Pacala Duclos' clone). In culture systems with forced renewal of internal atmosphere in culture flasks, Temporary Immersion System (TIS) and Constant Immersion System (CIS) with ...
Jova, MC., Kosky, RG., Cuellar, EE.
doaj
Vaso fermentador de bajo costo para la micropropagación masiva de jengibre
Agronomía Mesoamericana, 2007 Vaso fermentador de bajo costo para la micropropagación masiva de jengibre. La presente investigación se realizó en el Centro de Investigación en Biotecnología del Instituto Tecnológico de Costa Rica, durante el primer semestre del 2003 y tuvo como ...
Alejandro Hernández Soto +2 more
doaj +1 more source
A diffuse interface model for solid-liquid-air dissolution problems based on a porous medium theory [PDF]
, 2011 The underground rock may be dissolved by the flows of groundwater where the dissolution mainly happens at the liquid-solid interface. In many practical cases, the underground cavities are not occupied only by the water, but also the gas phase, e.g., air,
Quintard, Michel +3 more
core
Structural insights into an engineered feruloyl esterase with improved MHET degrading properties
FEBS Letters, EarlyView.A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa +5 more
wiley +1 more source
FEBS Letters, EarlyView.Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura +2 more
wiley +1 more source
Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation
FEBS Letters, EarlyView.Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz +11 more
wiley +1 more source
Degradation mechanism of the von Willebrand factor A2 domain by nattokinase
FEBS Letters, EarlyView.Nattokinase, a natto‐derived protease, exhibits potent antithrombotic effects. This study demonstrates that nattokinase directly cleaves the von Willebrand factor (vWF) A2 domain in vitro. Unlike the native regulator ADAMTS13, nattokinase degrades folded vWF independently of shear stress.
Ryuichi Hyakumoto +3 more
wiley +1 more source