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Detection of fish nocardiosis by loop-mediated isothermal amplification

Journal of Applied Microbiology, 2006
Loop-mediated isothermal amplification (LAMP) is a novel method that amplifies DNA with high specificity and rapidity under isothermal conditions. In this study, using the LAMP method, a protocol for detecting Nocardia seriolae which is a causative agent of fish nocardiosis, was designed.A set of four primers, two inner and two outer, were designed ...
T, Itano, H, Kawakami, T, Kono, M, Sakai
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Loop-Mediated Isothermal Amplification for the Detection of Plant Pathogens

2012
Loop-mediated isothermal amplification (LAMP) is a technique involving the use of four to six primers (two inner primers, two outer primers, and two loop primers) and the strand displacement activity of Bacillus subtilis-derived (Bst) DNA polymerase.
Lisa I, Ward, Scott J, Harper
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Loop‐Mediated Isothermal Amplification for Detection of Nucleic Acids

Current Protocols in Molecular Biology, 2014
AbstractSequence‐specific isothermal nucleic acid amplification techniques are ideally suited for use in molecular diagnostic applications because they do not require thermal cycling equipment and the reactions are typically fast. One of the most widely cited isothermal techniques is termed loop‐mediated isothermal amplification (LAMP).
Nathan A, Tanner, Thomas C, Evans
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[Loop-mediated isothermal amplification].

Nihon rinsho. Japanese journal of clinical medicine, 2007
We have developed the loop-mediated isothermal amplification (LAMP) method, an innovative gene amplification technique, which has the potential to become important in genetic testing. This method allows highly efficient amplification by a single enzyme at a constant temperature. It also has features such as high specificity, rapidity, and simplicity of
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Colorimetric strategies applicable for loop-mediated isothermal amplification

Journal of Microbiological Methods
In recent years, loop-mediated isothermal amplification (LAMP) has gained popularity for detecting various pathogen-specific genes due to its superior sensitivity and specificity compared to conventional polymerase chain reaction (PCR). The simplicity and flexibility of naked-eye detection of the amplicon make LAMP an ideal rapid and straightforward ...
Syaidatul Akmal, Saifuddin   +3 more
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In-Field Diagnostics Using Loop-Mediated Isothermal Amplification

2012
Loop-mediated isothermal amplification (LAMP) is a method for amplification and detection of target organisms which, unlike polymerase chain reaction, does not require thermal cycling. LAMP assays can be developed in the laboratory for subsequent deployment in the field, where the simplicity of isothermal amplification makes LAMP a suitable method for ...
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การพัฒนาวิธีการตรวจเนื้อสุกรปนเปื้อนในผลิตภัณฑ์อาหารโดยวิธี loop-mediated isothermal amplification

ได้ทำการพัฒนาการตรวจวิเคราะห์การปนเปื้อนเนื้อสุกรในผลิตภัณฑ์อาหารด้วยวิธี Loop-mediated isothermal amplification (LAMP) และวิธี LAMP-Dot Blotting โดยการออกแบบไพรเมอร์ 4 เส้นคือ Forward Inner Primer (FIP), Backward Inner Primer (BIP), Outer Primer F3 และ Outer Primer B3 ที่จำเพาะยีน cytochrome b และเพิ่มปริมาณดีเอ็นเอสุกรด้วยวิธี LAMP พบว่าสภาวะท ...
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Loop-Mediated Isothermal Amplification (LAMP)

2020
Alangar Ishwara Bhat, Govind Pratap Rao
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