Comparison of Different Approaches for Calculating LOD and LOQ in an HPLC-Based Analysis Method [PDF]
Pharmaceutical SciencesBackground: Sensitivity in the determination of the drug concentration is critical in pharmaceutical analysis. This research investigates several approaches for determining two sensitivity parameters, the Limit of Detection (LOD) and the Limit of ...
Raha Kaviani
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Structural insights into dsRNA processing by Drosophila Dicer-2–Loqs-PD
Nature, 2022 Abstract Small interfering RNAs (siRNAs) are the key components for RNA interference (RNAi), a conserved RNA-silencing mechanism in many eukaryotes 1,2 . In Drosophila , an RNase III enzyme Dicer-2 (Dcr-2), aided by its cofactor ...
Shichen Su +9 more
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Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila [PDF]
Nature Structural & Molecular Biology, 2009 In Drosophila melanogaster, the small interfering RNA (siRNA) pathway is triggered by exogenous double-stranded RNA (dsRNA) or upon viral infection. This pathway requires Dicer-2 (Dcr-2) in association with a dsRNA-binding protein (dsRBP) called R2D2.
João Trindade, Marques +5 more
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Loqs-PD and R2D2 define independent pathways for RISC generation in Drosophila [PDF]
Nucleic Acids Research, 2011 In Drosophila, siRNAs are classified as endo- or exo-siRNAs based on their origin. Both are processed from double-stranded RNA precursors by Dcr-2 and then loaded into the Argonaute protein Ago2. While exo-siRNAs serve to defend the cell against viruses, endo-siRNAs restrict the spread of selfish DNA in somatic cells, analogous to piRNAs in the germ ...
Hartig, Julia V., Förstemann, Klaus
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Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers
Nature Communications, 2023 AbstractSmall interference RNAs are the key components of RNA interference, a conserved RNA silencing or viral defense mechanism in many eukaryotes. In Drosophila melanogaster, Dicer-2 (DmDcr-2)-mediated RNAi pathway plays important roles in defending against viral infections and protecting genome integrity.
Ting Deng +6 more
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Citrate and Polyvinylpyrrolidone Stabilized Silver Nanoparticles as Selective Colorimetric Sensor for Aluminum (III) Ions in Real Water Samples [PDF]
, 2020 The use of silver nanoparticles stabilized with citrate and polyvinylpyrrolidone as a sensor for aluminum ions determination is proposed in this paper. These non-functionalized and specific nanoparticles provide a highly selective and sensitive detection
López Pérez, Germán +4 more
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Structural Basis of MicroRNA Biogenesis by Dicer-1 and Its Partner Protein Loqs-PB
Molecular Cell, 2022 SUMMARYIn animals and plants, Dicer enzymes collaborate with double-stranded RNA-binding proteins to convert precursor-microRNAs (pre-miRNAs) into miRNA duplexes. We report six cryo-EM structures of Drosophila Dicer-1 and its partner Loqs-PB. The structures show Dicer-1•Loqs-PB (1) before binding pre-miRNA, (2) after binding and in a catalytically ...
Karina Jouravleva +6 more
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The status of Fusarium mycotoxins in Sub-Saharan Africa : a review of emerging trends and post-harvest mitigation strategies towards food control [PDF]
, 2017 Fusarium fungi are common plant pathogens causing several plant diseases. The presence of these molds in plants exposes crops to toxic secondary metabolites called Fusarium mycotoxins. The most studied Fusarium mycotoxins include fumonisins, zearalenone,
Atanda, Olusegun Oladimeji +3 more
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Infant exposure to lithium through breast milk
European Psychiatry, 2021 Introduction Women who take lithium during pregnancy and continue after delivery may opt to breastfeed, formula feed, or mix these options. Objectives To evaluate the neonatal lithium plasma concentrations and nursing infant outcomes based on these ...
M.L. Imaz +5 more
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Neurobehavioral function and low-level exposure to brominated flame retardants in adolescents : a cross-sectional study [PDF]
, 2012 Background: Animal and in vitro studies demonstrated a neurotoxic potential of brominated flame retardants, a group of chemicals used in many household and commercial products to prevent fire.
Baeyens, Willy +12 more
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