Results 61 to 70 of about 2,165 (235)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Development of Copper Oxide Thin Film for Lossy Mode Resonance-Based Optical Fiber Sensor
Proceedings, 2018 In this work we present the study of copper(II) oxide thin films for the fabrication of lossy mode resonance-based (LMR) optical fiber sensors. This material has proven to be capable of generating such resonances with a promising result.
Aritz Ozcariz
doaj +1 more source
FEBS Open Bio, EarlyView.Evolutionary analysis across 32 placental mammals identified positive selection at residues H148 and W149 in the immune receptor FcγR1. Ancestral reconstruction combined with molecular dynamics simulations reveals how these mutations may influence receptor structure and dynamics, providing insight into the evolution of antibody recognition and immune ...
David A. Young +7 more
wiley +1 more source
Fiber-optic biosensor based on lossy mode resonances
Sensors and Actuators B: Chemical, 2012 Abstract In this work, a novel fiber-optic immunoglobulin G biosensor based on lossy mode resonances is proposed. This physical phenomenon is obtained by depositing a PAH/PSS layer-by-layer thin-film on a cladding removed optical fiber. A reflective configuration is used to track the generation and response of the electromagnetic resonance in the ...
Socorro Leránoz, Abián Bentor +4 more
openaire +3 more sources
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
Lossy-mode-resonance sensor based on perovskite nanomaterial with high sensitivity
Optics Express, 2021 Lossy-mode-resonance (LMR) is a surface plasmon resonance (SPR)-analogue optical phenomenon, which is sensitive to the surrounding environment variations and can be considered as an important detection signal in biochemical sensors. Compared with the SPR sensor which can only operate under transverse magnetic (TM)-polarized light, the LMR sensor shows ...
Leiming Wu, Yuanjiang Xiang, Yuwen Qin
openaire +2 more sources
FEBS Open Bio, EarlyView.NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks +4 more
wiley +1 more source
GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma
FEBS Open Bio, EarlyView.GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain +11 more
wiley +1 more source
Lossy Mode Resonance Sensors Based on Planar Waveguides: Theoretical and Experimental Comparison
IEEE Photonics JournalLossy mode resonance (LMR) has garnered significant attention in sensor applications. LMR was primarily explored in fiber-based systems, however, there has been a recent upsurge in its application within planar waveguides.
Edvins Letko +2 more
doaj +1 more source
TM02 Quarter-Mode Substrate-Integrated Waveguide Resonator for Dual Detection of Chemicals
Sensors, 2018 The detection of multiple fluids using a single chip has been attracting attention recently. A TM02 quarter-mode substrate-integrated waveguide resonator designed at 5.81 GHz on RT/duroid 6010LM with a return loss of 13 dB and an unloaded quality factor ...
Ahmed Salim, Sungjoon Lim
doaj +1 more source