Results 81 to 90 of about 862,186 (311)

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Numerical modeling of InGaAs/AlGaAs heterostructure for short-wave infrared nBn photodetectors

open access: yesJournal of the European Optical Society-Rapid Publications
nBn infrared photodetectors have emerged as a strong alternative to conventional pn and pin-based structures due to their low dark current, fast response, and suppression of Shockley–Read–Hall (SRH) generation pathways. In this study, an InP-based InGaAs/
Tok Çağrı   +5 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Directed evolution of enzymes at the crossroads of tradition and innovation

open access: yesFEBS Open Bio, EarlyView.
An iterative cycle of data‐driven enzyme optimization comprising four stages: genetic diversification of a template enzyme, expression of protein variants, high‐throughput evaluation, and machine‐learning‐guided redesign of the next variant library.
Maria Tomkova   +2 more
wiley   +1 more source

Low noise pavement characterization through TCN analysis

open access: yes
The use of low-noise pavements is a valid solution to reduce road traffic noise at source. Further studies are needed to optimize their design and ensure their durability.
MORO, Antonino   +4 more
core   +1 more source

Low-Frequency Noise Characteristics of AlGaAs/InGaAs Pseudomorphic HEMTs [PDF]

open access: yes, 2001
The low-frequency noise of InGaAs pseudomorphic HEMTs fabricated on GaAs substrate was studied. The dependence of the noise spectral density on the gate voltage indicates that the channel of the device dominates the low-frequency noise.
MIZUTANI, Takashi   +3 more
core  

Hyperosmotic stress‐induced redistribution of pre‐mRNA cleavage factor I subunits is associated with shifts in alternative polyadenylation

open access: yesFEBS Open Bio, EarlyView.
Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya   +2 more
wiley   +1 more source

Ultralow-Noise Chopper Amplifier for Seafloor E-Field Measurement

open access: yesSensors
The seafloor E-field signal is extremely weak and difficult to measured, even with a high signal-to-noise ratio. The preamplifier for electrodes is a key technology for ocean-bottom electromagnetic receivers.
Sixuan Song, Kai Chen
doaj   +1 more source

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress

open access: yesFEBS Open Bio, EarlyView.
Miniaturized flow chip platform enabling continuous perfusion and longitudinal multiphoton 3D imaging of vascular smooth muscle cell constructs under physiological flow. Brightfield imaging guides region selection, while CellTracker Green and mRuby‐labeled fetuin‐A visualize cells and mineral deposition, respectively. Magnesium supplementation markedly
Vytautas Kučikas   +6 more
wiley   +1 more source

Life-cycle costs and infrastructural investments induced by unconventional low-noise aircraft

open access: yes, 2015
The environment challenge and the expected growth of the market demand impose a technology breakthrough within the aeronautical industry. Although several innovative configurations of airplanes have been analyzed, none of them has been yet chosen as the ...
CENTRACCHIO, FRANCESCO   +2 more
core  

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