In silico and in vitro exploration of a tyrosinase for biocatalytic production of catechols
FEBS Open Bio, EarlyView.Tyrosinase from Ralstonia pseudosolanacearum is a promising biocatalyst for producing valuable catechols from monophenol substrates. This tyrosinase is uniquely suited to this due to its high monophenolase : diphenolase ratio. We combined in silico docking and in vivo kinetic characterisation of this tyrosinase with 11 industrially relevant monophenols,
James Britton +6 more
wiley +1 more source
Finite-Time Thermodynamics of Battery Discharging: Power-Efficiency Trade-Off and Optimization. [PDF]
Entropy (Basel)Liu RH, Lin YQ, Ma YH.
europepmc +1 more source
Adenosine triphosphate as a modulator of protein interactions and stability
FEBS Open Bio, EarlyView.ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley +1 more source
Theoretical estimates on the expected number of mutations needed to reconstruct clonal lineage trees. [PDF]
Bioinform AdvBristy NA, Schwartz R.
europepmc +1 more source
FEBS Open Bio, EarlyView.We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen +5 more
wiley +1 more source
Protocol for quantifying miRNA trafficking across the endosomal membrane
FEBS Open Bio, EarlyView.An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh +2 more
wiley +1 more source
Near-Frustration-Free Electronic Structure Hamiltonian Representations and Lower-Bound Certificates. [PDF]
J Chem Theory ComputRubin NC, Low GH, DePrince Iii AE.
europepmc +1 more source
Adversarial dynamical systems characterize when data-driven learning succeeds or fails. [PDF]
Nat CommunColbrook MJ, Mezić I, Stepanenko A.
europepmc +1 more source
On the minimum number of non-monochromatic simplices for Sperner labelings of a regular triangulation. [PDF]
PLoS OneCalvo Pascual LÁ +4 more
europepmc +1 more source