Results 161 to 170 of about 26,398 (197)
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Simplified assay for lysyl oxidase activity
Analytical Biochemistry, 1977Abstract A simplified method for the assay of lysyl oxidase activity was developed. The method is based on the measurement of tritiated water released by enzyme action from labeled protein-bound lysine and hydroxylysine. Trichloroacetic acid (TCA) supernates of the incubation mixtures are passed through small Dowex 50 (H+) columns and the effluents ...
J, Melet, G D, Vianden, B N, Bachra
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Lysyl oxidase expression in bronchogenic carcinoma
The American Journal of Surgery, 2005Lysyl oxidase catalyzes a key step in the cross-linking of collagen and elastin in the extracellular matrix. Recent studies have documented differential lysyl oxidase expression in the stromal reaction to colon, breast, prostate, and lung cancer. The present study was undertaken to test the hypothesis that lysyl oxidase mRNA and protein expression ...
Amy R, Woznick +8 more
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Properties and Function of Lysyl Oxidase
American Journal of Respiratory Cell and Molecular Biology, 1991Lysyl oxidase catalyzes the oxidation of peptidyl lysine to alpha-aminoadipic-delta-semialdehyde, the precursor to the covalent crosslinkages that stabilize fibers of elastin and collagen. This enzyme contains both copper and a carbonyl cofactor consistent with an o-quinone.
H M, Kagan, P C, Trackman
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Lysyl oxidase and adipose tissue dysfunction
Metabolism, 2018Lysyl oxidase (LOX) is an enzyme crucial for collagen fibre crosslinking and thus for fibrosis development. Fibrosis is characterised by a surplus of collagen fibre accumulation and is amongst others also a feature of obesity-associated dysfunctional adipose tissue (AT) which has been linked with type 2 diabetes. We hypothesised that in type 2 diabetes
Pastel, E. +9 more
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Monoclonal Antibodies to Human Lysyl Oxidase
Collagen and Related Research, 1986Hybridoma antibodies against human lysyl oxidase were produced by fusing Sp. 2.0-Ag 14 myeloma cells with spleen cells from mice hyperimmunized with lysyl oxidase isolated from umbilical cords. Hybridomas positive by enzyme-linked immunosorbent assay (ELISA) for human lysyl oxidase were cloned by the dilution method.
Burbelo, Peter D. +2 more
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2001
Publisher Summary The chapter discusses the four quinone cofactors that use reactive carbonyls to catalyze amine or alcohol oxidation. These includes, trihydroxyphenylalanine quinone (TPQ) found in copper amine oxidases (CAOs) and lysine tyrosylquinone (LTQ) found in lysyl oxi- dase (LOX) are discussed in this chapter.
J E, Dove, J P, Klinman
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Publisher Summary The chapter discusses the four quinone cofactors that use reactive carbonyls to catalyze amine or alcohol oxidation. These includes, trihydroxyphenylalanine quinone (TPQ) found in copper amine oxidases (CAOs) and lysine tyrosylquinone (LTQ) found in lysyl oxi- dase (LOX) are discussed in this chapter.
J E, Dove, J P, Klinman
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Frontiers in Bioscience, 2008
Lysyl oxidase (LOX) plays a pivotal role in extracellular matrix (ECM) maturation. Furthermore, novel biological functions has been ascribed to LOX, among them cell differentiation, migration, transformation and regulation of gene expression. In this context, it has been suggested that abnormalities of LOX expression could underlie the development of ...
Javier F, Alcudia +5 more
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Lysyl oxidase (LOX) plays a pivotal role in extracellular matrix (ECM) maturation. Furthermore, novel biological functions has been ascribed to LOX, among them cell differentiation, migration, transformation and regulation of gene expression. In this context, it has been suggested that abnormalities of LOX expression could underlie the development of ...
Javier F, Alcudia +5 more
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Crystallization ofPichia pastorislysyl oxidase
Acta Crystallographica Section D Biological Crystallography, 2002A copper-containing amine oxidase (PPLO) from the yeast Pichia pastoris has been purified and crystallized in two forms. PPLO is a glycoprotein. The molecular mass from SDS-polyacrylamide gels is 112 kDa, consistent with 20% glycosylation by weight (the calculated molecular weight of the polypeptide is 89.7 kDa).
Mihwa, Lee +9 more
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Lysyl oxidases: A novel multifunctional amine oxidase family
2001Lysyl oxidase (LOX), a copper-containing amine oxidase, belongs to a heterogeneous family of enzymes that oxidize primary amine substrates to reactive aldehydes. LOX has been traditionally known for one function, the extracellular catalysis of lysine-derived cross-links in fibrillar collagens and elastin.
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