Polysaccharide degradation by lytic polysaccharide monooxygenases [PDF]
Current Opinion in Structural Biology, 2019 The discovery of oxidative cleavage of glycosidic bonds by enzymes currently known as lytic polysaccharide monooxygenases (LPMOs) has had a major impact on our current understanding of the enzymatic conversion of recalcitrant polysaccharides such as ...
Røhr, Åsmund +11 more
core +7 more sources
Enzymatic upgrading of nanochitin using an ancient lytic polysaccharide monooxygenase [PDF]
Communications Materials, 2022 Enzymes are effective at chemically converting low-value biomass to technological materials. Here, an ancestral lytic polysaccharide monooxygenase enzyme is used to synthesize chitin nanocrystals, which are used as a matrix for cell growth and to create ...
Leire Barandiaran +8 more
doaj +2 more sources
Improved spectrophotometric assay for lytic polysaccharide monooxygenase [PDF]
Biotechnology for Biofuels, 2019 Background The availability of a sensitive and robust activity assay is a prerequisite for efficient enzyme production, purification, and characterization.
Erik Breslmayr +6 more
doaj +4 more sources
A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains
Biotechnology for Biofuels, 2019 Background Efforts to develop efficient lignocellulose-degrading enzymatic preparations have led to the relatively recent discovery of a new class of novel cellulase boosters, termed lytic polysaccharide monoxygenases (LPMOs).
Pooja Dixit +5 more
doaj +2 more sources
A colorimetric assay to rapidly determine the activities of lytic polysaccharide monooxygenases [PDF]
Biotechnology for Biofuels, 2018 Background Lytic polysaccharide monooxygenase (LPMOs) are enzymes that catalyze the breakdown of polysaccharides in biomass and have excellent potential for biorefinery applications.
Damao Wang +4 more
doaj +2 more sources
Multiscale Modelling of Lytic Polysaccharide Monooxygenases [PDF]
ACS Omega, 2017 Lytic polysaccharide monooxygenase (LPMO) enzymes have attracted considerable attention owing to their ability to enhance polysaccharide depolymerization, making them interesting with respect to production of biofuel from cellulose.
Hedegård, Erik +8 more
core +5 more sources
Structural Dynamics of Lytic Polysaccharide Monooxygenase during Catalysis [PDF]
Biomolecules, 2020 Lytic polysaccharide monooxygenases (LPMOs) are industrially important oxidoreductases employed in lignocellulose saccharification. Using advanced time-resolved mass spectrometric techniques, we elucidated the structural determinants for substrate ...
Frantisek Filandr +6 more
doaj +3 more sources
A fast and sensitive activity assay for lytic polysaccharide monooxygenase
Biotechnology for Biofuels, 2018 Background Lytic polysaccharide monooxygenases (LPMO) release a spectrum of cleavage products from their polymeric substrates cellulose, hemicellulose, or chitin.
Erik Breslmayr +7 more
doaj +3 more sources
Structural and electronic determinants of lytic polysaccharide monooxygenase reactivity on polysaccharide substrates [PDF]
Nature Communications, 2017 Copper-dependent lytic polysaccharide monooxygenases (LPMOs) oxidatively cleave polysaccharides. Here the authors present a structure-function characterization of fungal LPMOs, showing that a particular LPMO cleaves xylan using a mechanism that involves ...
T. J. Simmons +14 more
doaj +16 more sources
Histidine oxidation in lytic polysaccharide monooxygenase. [PDF]
J Biol Inorg Chem, 2023 Abstract The lytic polysaccharide monooxygenases (LPMOs) comprise a super-family of copper enzymes that boost the depolymerisation of polysaccharides by oxidatively disrupting the glycosidic bonds connecting the sugar units.
Torbjörnsson M +3 more
europepmc +5 more sources