Results 121 to 130 of about 13,887 (165)

Radiolabeling of M13 bacteriophages for in vivo imaging

open access: yes
Trommer, J.   +9 more
openaire   +1 more source

Replication of bacteriophage M13

Journal of Molecular Biology, 1977
The origin for bacteriophage M13 single-strand synthesis has been localized by two different experimental approaches: (1) localization of the discontinuity in late life-cycle RFII ‡ , one of the initial product molecules during single-strand synthesis, and (2) determination of a gradient of radioactive label in RFI pulselabeled during single-strand ...
Lorraine Lica, Dan S. Ray
openaire   +2 more sources

Plating Bacteriophage M13

Cold Spring Harbor Protocols, 2006
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Joseph, Sambrook, David W, Russell
openaire   +2 more sources

Plating Bacteriophage M13

Cold Spring Harbor Protocols, 2017
A plaque of bacteriophage M13 derives from infection of a single bacterium by a single virus particle. The progeny particles infect neighboring bacteria, which, in turn, release another generation of daughter virus particles. If the bacteria are growing in semisolid medium (e.g., containing agar or agarose), then the diffusion of the progeny particles ...
Michael R, Green, Joseph, Sambrook
openaire   +2 more sources

Growing Bacteriophage M13 in Liquid Culture

Cold Spring Harbor Protocols, 2017
Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Michael R, Green, Joseph, Sambrook
openaire   +2 more sources

M13 Bacteriophages as Bioreceptors in Biosensor Device

2019
New recognition probes sensible, specific and robust is one of the major problems of biosensor assay. Detection biosensors has utilized antibodies or enzymes as bioreceptors; however, these have numerous disadvantages of limited binding sites and physico-chemical instabilities, can negatively affect capture and detection of target in diagnostic device.
De Plano, Laura M.   +6 more
openaire   +3 more sources

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