Results 171 to 180 of about 23,200 (213)

Growing Bacteriophage M13 in Liquid Culture

Cold Spring Harbor Protocols, 2006
Stocks of bacteriophage M13 are usually grown in liquid culture. The infected bacteria do not lyse but, instead, grow at a slower than normal rate to form a dilute suspension. The inoculum of bacteriophage is almost always a freshly picked plaque or a suspension of bacteriophage particles obtained from a single plaque, as described here. Infected cells
Joseph, Sambrook, David W, Russell
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M13 bacteriophage displaying disulfide-constrained microproteins

Gene, 1993
A display-phage library (TN2), displaying an 18-residue peptide fused to coat protein III, represents a collection of up to 8.55 x 10(6) peptides encoded by only 1.68 x 10(7) DNA sequences. Each displayed peptide has two fixed cysteine residues (allowing disulfide formation) and six variegated residues, four between the cysteines and one either side of
M A, McLafferty, R B, Kent, R C, Ladner, W, Markland   +3 more
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Genetic control of bacteriophage M13 DNA synthesis

Journal of Molecular Biology, 1968
Abstract In the filamentous single-stranded DNA phage M13, the DNA replicates via the three steps shown in Figure 1: first, conversion of the infecting single-stranded molecule to a double-stranded replicative form; then, production of a pool of RF † molecules; finally, synthesis of progeny single strands.
D, Pratt, W S, Erdahl
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M13 bacteriophage production for large-scale applications

Bioprocess and Biosystems Engineering, 2014
Bacteriophage materials have the potential to revolutionize medicine, energy production and storage, agriculture, solar cells, optics and many other fields. To fulfill these needs, this study examined critical process parameters during phage propagation to increase phage production capability. A representative scale-down system was created in tube spin
Christopher M, Warner, Natalie, Barker, Seung-Wuk, Lee, Edward J, Perkins   +3 more
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Structural characterization of bacteriophage M13 solubilization by amphiphiles

Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 2002
The structural properties of bacteriophage M13 during disassembly were studied in different membrane model systems, composed of a homologue series of the detergents sodium octyl sulfate, sodium decyl sulfate, and sodium dodecyl sulfate. The structural changes during phage disruption were monitored by spin-labeled electron spin resonance (ESR) and ...
Stopar, D., Spruijt, R.B., Wolfs, C.J.A.M., Hemminga, M.A.   +3 more
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M13 Bacteriophages as Bioreceptors in Biosensor Device

2019
New recognition probes sensible, specific and robust is one of the major problems of biosensor assay. Detection biosensors has utilized antibodies or enzymes as bioreceptors; however, these have numerous disadvantages of limited binding sites and physico-chemical instabilities, can negatively affect capture and detection of target in diagnostic device.
De Plano, Laura M., Franco, Domenico, Rizzo, Maria Giovanna, Crea, Sara, Messina, Grazia M. L., Marletta, Giovanni, Guglielmino, Salvatore P. P.   +6 more
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Cloning SV40 HindIII restriction fragments into M13 bacteriophage

Gene, 1982
Abstract The six Hind III restriction fragments of the simian virus 40 (SV40) genome were cloned into the bacteriophage M13mp5 vector to generate strand-specific hybridization probes.
K, Dixon, S W, Barnett, C K, Lau, P C, Stacks   +3 more
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Inhibition of Bacteriophage M13 Replication with Esterified Milk Proteins

Journal of Agricultural and Food Chemistry, 2006
Esterified milk proteins [methylated (Met) or ethylated (Et) alpha-lactalbumin (ALA), beta-lactoglobulin (BLG), and beta-casein (BCN)], unmodified native milk proteins, and native basic proteins (calf thymus histone and hen egg white lysozyme) were tested for their antiviral activity against the bacteriophage M13 and for their influence on its ...
Sitohy, Mahmoud Zaki, Chobert, Jean-Marc, Karwowska, Urszula, Gozdzicka-Josefiak, Anna, Haertlé, Thomas   +4 more
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M13 Bacteriophage-Assisted Biomineralization of Copper Sulfide

MRS Proceedings, 2012
ABSTRACTCombinatorial phage display with a pVIII library of M13 bacteriophage was used to identify a peptide sequence capable of recognition and mineralization of copper sulfide. The six sequences isolated from the final biopanning round were rich in basic, hydrophobic, and polar amino acids compared to the phage display library.
Mohammed Shahriar Zaman, Elaine D. Haberer   +1 more
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Molecular basis of the am8H1 lesion in bacteriophage M13

Virology, 1979
Abstract The nucleotide sequence of the mutant region of am8H1, the only conditional lethal mutant in gene VIII of the filamentous phages, has been determined and compared to the wild-type sequence. The amber mutation lies adjacent to the codons specifying the cleavage site for the procaryotic signal peptidase (1).
J D, Boeke, P, Model
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