Verification of accuracy of qPCR method for intravital diagnostics of Macrorhabdus ornithogaster in avian droppings [PDF]
The objective of this study was to verify the accuracy of the quantitative PCR (qPCR) method for in vivo detection of Macrorhabdus ornithogaster. A total of 100 faecal and gastric mucosa samples from avian cadavers were investigated, using cytological ...
L Vrbasova +3 more
doaj +5 more sources
New Diagnostic Insights for Macrorhabdus ornithogaster Infection [PDF]
ABSTRACT The aim of this study was to propose the use of a new rapid and user-friendly diagnostic tool for the detection of Macrorhabdus ornithogaster infection in birds. The current report focuses on the diagnostic feasibility of different methods, with particular emphasis on the application ...
Luca Borrelli +2 more
exaly +7 more sources
Macrorhabdosis is a chronic gastric debilitating disease causing loses in most bird’s species. Therefore, this article was designed to outline avian macrorhabdosis disease regarding the incidence, susceptibility, transmission, clinical picture ...
Wafaa Abd El-Ghany
doaj +4 more sources
Megabacteria (Macrorhabdus ornithogaster) em psitacídeos mantidos em estabelecimentos comerciais do município de Uberaba – MG [PDF]
A megabacteriose é uma doença que acomete algumas espécies de aves, tendo como agente etiológico Macrorhabdus ornithogaster, um microrganismo Gram positivo de forma bacilar e com características de fungo, sendo classificado como fungo ascomiceto ...
Isabella Hercos de Paula +6 more
doaj +4 more sources
Analysis for the diagnostic accuracy of PCR detection of Macrorhabdus in companion birds [PDF]
Background: Macrorhabdus ornithogaster, a yeast-like fungus, has the potential to infect various bird species, including companion birds. Although birds infected with M. ornithogaster may often remain asymptomatic, the infection can develop into chronic
Shoko Kanno, Yusuke Matsumoto
doaj +2 more sources
Molecular identification and phylogenetic analysis of Macrorhabdus ornithogaster based on the 18S rRNA gene in companion birds of Tehran, Iran. [PDF]
Macrorhabdus ornithogaster (MO) is an infectious yeast which can cause acute gastric disturbances in birds. It has a worldwide distribution with a broad host-range of bird species.Molecular identification and phylogenetic analysis of MO based on the 18S rRNA gene in companion birds of Iran.A total of 54 stool samples were taken from birds (10 species ...
Hamian F +3 more
europepmc +3 more sources
Validation of the usefulness of 26S rDNA D1/D2, internal transcribed spacer, and intergenic spacer 1 for molecular epidemiological analysis of Macrorhabdus ornithogaster. [PDF]
Macrorhabdus ornithogaster (MO) is an infectious fungus that causes gastric damage in birds. In this study, we established nested and seminested polymerase chain reaction (PCR) methods that specifically amplify the domain D1/D2 region (D1/D2) of 26S ribosomal DNA (rDNA), internal transcribed spacer (ITS) of rDNA, and intergenic spacer (IGS) 1 region ...
Kojima A +5 more
europepmc +4 more sources
An outbreak of severe Macrorhabdus ornithogaster infection in common canaries (Serinus canarius domesticus), Molecular and pathological assay [PDF]
Infection by Macrorhabdus ornithogaster (MO) formerly called Megabacteriosis has been diagnosed as the cause of depression, wasting, regurgitation, fluffed feathers, lethargy, and soft watery bulky feces and also the cause of mortality in a flock of ...
Jamshid Razmyar +2 more
doaj +2 more sources
Macrorhabdus ornithogaster in ostrich, rhea, canary, zebra finch, free range chicken, turkey, guinea-fowl, columbina pigeon, toucan, chuckar partridge and experimental infection in chicken, japanese quail and mice [PDF]
Since 2000, Macrorhabdus ornithogaster "megabacteriosis" has been diagnosed in the avian diseases laboratory in a diversity of avian species and varied spectrum of disease. The disease in some species (chickens, turkeys, guinea fowls) was clinically characterized by emaciation, prostration, loss of appetite, cachexia and death, with a typically chronic
N R S Martins, R A Assis, Assis R A
exaly +6 more sources
Abstract Objective To develop and validate a quantitative PCR (qPCR) assay for detecting Macrorhabdus ornithogaster (MO) and reproducibly culture MO from infected budgerigars (Melopsittacus undulatus). Methods A TaqMan qPCR assay targeting a 94-bp segment of MO 18S rRNA was evaluated for limit of detection, dynamic range, intra-assay variability ...
Laura Adamovicz +2 more
exaly +3 more sources

