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Juvenile hormone-controlled synthesis of female-specific protein in the cockroach Leucophaea maderae

Archives of Biochemistry and Biophysics, 1971
Application of 1 μg of the all-trans methyl (±)-12,14-dihomojuvenate induces the de novo synthesis of a female-specific protein at a rate similar to that found in unoperated egg-maturing females. JH also appears to stimulate an increased synthesis of the non-sex-specific proteins.
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Ovarian maturation in Leucophaea maderae: Juvenile hormone regulation of thymidine uptake into follicle cell DNA

Journal of Insect Physiology, 1980
Abstract Our research demonstrates that juvenile hormone (JH I) stimulates thymidine incorporation into ovarian follicle cell DNA in the ovoviviparous cockroach, Leucophaea maderae. A rapid, quantitative method for monitoring 3H-thymidine incorporation into ovarian DNA, in vitro, is described. Cultured ovarian tissue from L.
John K. Koeppe, Susan E. Wellman
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Juvenile Hormone Induced Biosynthesis of Vitellogenin in Leucophaea maderae from Large Precursor Polypeptides

1976
Despite the extensive research in recent years on insect juvenile hormone (JH), its mode of action at the molecular level is still not understood. One reason for this gap in our knowledge is that the usual morphological assays of JH action do not lend themselves readily to analysis by molecular biological tools. For this reason, we have chosen to study
J. K. Koeppe, J. Ofengand
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Juvenile hormone control of female specific protein synthesis in Leucophaea maderae, Schistocerca vaga, and Sarcophaga bullata

Journal of Insect Physiology, 1971
Abstract In Schistocerca vaga a female specific protein is present in the haemolymph during egg maturation. This protein is absent from the haemolymph before the start of ovarian development, in females allatectomized before the start of ovarian development, and in males of any age.
F. Engelmann, L. Hill, J.L. Wilkens
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The induction of yolk protein synthesis in the fat body of an insect, Leucophaea maderae, by an analog of the juvenile hormone

Developmental Biology, 1969
Abstract During the time that eggs are developing, the fat body from female Leucophaea maderae synthesizes a protein with characteristics similar to a subunit of the major yolk protein. Synthesis does not occur or is much reduced in fat bodies from males, nymphs, and abdomens amputated from adult females before the onset of egg development.
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Phospholipid synthesis in the fat body endoplasmic reticulum during primary and secondary juvenile hormone stimulation of vitellogenesis inLeucophaea maderae

Wilhelm Roux's Archives of Developmental Biology, 1984
Juvenile hormone (JH) treatment coordinately stimulated the dose-dependent synthesis of vitellogenin and endoplasmic reticulum (ER) membrane phospholipids in fat body cells from allatectomized adult females ofLeucophaea maderae. Animals were pulse-labeled in vivo with [32P] to simultaneously measure the rates of synthesis of the phosphorylated subunits
Guy, Della-Cioppa, Franz, Engelmann
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Juvenile hormone regulation of phospholipid synthesis in the endoplasmic reticulum of vitellogenic fat body cells from Leucophaea maderae

Insect Biochemistry, 1984
Abstract The endoplasmic reticulum (ER) from fat body cells of Leucophaea maderae was isolated as a purified population of rough and smooth-surfaced microsomal vesicles which was highly enriched in NADH-cytochrome c reductase activity. Phosphatidylcholine (PC) was the major structural-phospholipid of the ER membrane and it accounted for 58% of ...
Guy Della-Cioppa, Franz Engelmann
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Synthesis and secretion of juvenile hormone binding protein by fat body from the cockroach Leucophaea maderae: Protein a immunoassay

Insect Biochemistry, 1987
Fat body maintained in vitro from adult female Leucophaea maderae synthesizes and secretes at least two peptides (275 and 220 kDa by SDS-PAGE) that can be photoaffinity labelled with the juvenile hormone analog 10-[10,11-3H]epoxyfarnesyl diazoacetate ([3H]EFDA).
John K. Koeppe   +3 more
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Changes in lipid content during the reproductive cycle of Leucophaea maderae and effects of the juvenile hormone on lipid metabolism in vitro

Comparative Biochemistry and Physiology, 1967
1. 1. Lipid is stored in both the ovaries and fat body of female Leucophaea maderae during the 20-day period of oogenesis and is a major substrate for the developing embryos during the ensuing 2 months of embryogenesis. 2. 2. The lipid picture for the period of embryogenesis is characterized by a decreasing content of embryonic triglyceride and ...
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Changes in follicle cell morphology, ovarian protein synthesis and ovarian DNA synthesis during oöcyte maturation in Leucophaea maderae: Role of juvenile hormone

Journal of Insect Physiology, 1981
Abstract Changes in follicle cell morphology were correlated with changes in rates of protein synthesis and DNA synthesis by the ovary during ovarian maturation in Leucophaea maderae. During the vitellogenic period of oocyte development, which lasts approx, 15 days, morphological changes in the follicle cells are accompanied by moderate rates of ...
John K. Koeppe   +2 more
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