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Centro Andaluz de Documentación e Información Juvenil
La Colección GEPOJU - CENID del IIGG reúne un amplio archivo con documentos históricos elaborados durante el periodo 1980-2006 en Argentina, América Latina y el Caribe y, España, por: Organismos Gubernamentales de Juventud; Organismos Gubernamentales NO ...
Centro de Documentación e Información Juvenil
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Juvenile hormone-induced biosynthesis of vitellogenin in Leucophaea maderae
Archives of Biochemistry and Biophysics, 1976Abstract Isolated abdomens of virgin female Leucophaea maderae, a South American cockroach, could be induced by a single injection of juvenile hormone I to synthesize vitellogenin. Synthetic capacity was assayed by removal of the fat body at various times after induction and incubation in organ culture with radioactive leucine for 5 h.
J, Koeppe, J, Ofengand
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Assay and identification of juvenile hormone binding proteins in leucophaea maderae
Molecular and Cellular Endocrinology, 1983We modified a binding assay using polyethylene glycol (PEG) to precipitate bound hormone. Optimum precipitation occurred when reaction mixtures were incubated with 10-40% PEG and 1.25-2.5 mg/ml gamma-globulins for 2-90 min at 4 or 23 degrees C. Results from this assay and from the dextran-coated charcoal assay were similar.
G E, Kovalick, J K, Koeppe
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Follicle cell polyploidy in Leucophaea maderae: Regulation by juvenile hormone
Journal of Insect Physiology, 1985Abstract Microspectrophotometric analysis of Feulgen-stained nuclei of the terminal follicle cells in the cockroach Leucophaea maderae showed that during maturation the follicle cells became polyploid. In virgin females, the follicle cell nuclei were diploid. After mating, and during vitellogenesis, the ploidy of the follicle cells increased from 2 C
Margot C. LaPointe +2 more
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Insect Biochemistry, 1984
Two JH carboxylesterases were isolated from haemolymph of vitellogenic females by anion exchange chromatography. The high degree of purification achieved by this one step procedure is inferred by the absence of any protein stainable band (Coomassie blue or silver stain) on native polyacrylamide gels, yet enzyme activity was detectable in eluates of ...
Sonny Gunawan, Franz Engelmann
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Two JH carboxylesterases were isolated from haemolymph of vitellogenic females by anion exchange chromatography. The high degree of purification achieved by this one step procedure is inferred by the absence of any protein stainable band (Coomassie blue or silver stain) on native polyacrylamide gels, yet enzyme activity was detectable in eluates of ...
Sonny Gunawan, Franz Engelmann
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Relationship of hemolymph juvenile hormone-binding protein to lipophorin in Leucophaea maderae
Insect Biochemistry, 1988Abstract Monoclonal antibodies (MAb) specific for the hemolymph 220 kD juvenile hormone-binding protein (JHBP) of the cockroach, Leucophaea maderae, were used to further characterize the JHBP. Spleen cells, from mice immunized with semipurified hemolymph JHBP, were fused with myeloma cells to produce hybridomas secreting antibodies specific for the ...
Richard C. Rayne, John K. Koeppe
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