Results 141 to 150 of about 9,996 (183)
Regulation of Autophagy Machinery in Magnaporthe oryzae
International Journal of Molecular Sciences, 2022 Plant diseases cause substantial loss to crops all over the world, reducing the quality and quantity of agricultural goods significantly. One of the world’s most damaging plant diseases, rice blast poses a substantial threat to global food security.
Fu-Cheng Lin, Xueming Zhu, Nida Asif
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Characterizing the Secretion Systems of Magnaporthe oryzae
2021Pharmacological approaches have made a tremendous impact on the field of microbial secretion systems. This protocol describes the inhibition of Golgi-dependent secretion in Magnaporthe oryzae though brefeldin A (BFA) treatment. State-of-the-art live-cell imaging allows tracking secreted proteins in their secretion pathways.
Ely, Oliveira-Garcia, Barbara, Valent
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Controllable Bypass Suppression in Magnaporthe oryzae
2021Evolutionary adaptation of living organisms is commonly thought to be the result of processes that have taken place over long periods of time. By contrast, we found that the filamentous rice blast fungus Magnaporthe oryzae rapidly suppresses the osmosensitive "loss of function" (lof) phenotype in knockout mutants of the high-osmolarity glycerol (HOG ...
Stefan, Jacob, Katharina, Bersching
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Methods to Study Autophagocytosis in Magnaporthe oryzae
2021Autophagy is an evolutionarily conservative biological process in eukaryotes. Since the lysosomes were discovered by De Duve in the 1950s, autophagy has been studied for more than half a century and the mechanism of autophagy process has been discovered in many model organisms.
Lin, Li +3 more
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Functional analysis of MoSnf7 in Magnaporthe oryzae
Fungal Genetics and Biology, 2018Snf7 is the core subunit protein of the yeast endosomal sorting complex required for transport (ESCRT) complex, which plays important roles in endocytosis and autophagy. In this study, we characterized MoSnf7 in Magnaporthe oryzae, a homolog of yeast Snf7, the core protein of ESCRT-III subcomplex.
Jie Cheng +3 more
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Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae
2021The quality and consistency in every sample preparation procedure is crucial for any scientific output. Therefore, it is of utmost importance to have easy, economic, and robust sample preparation protocols. Here, we describe a simple and robust bottom-up proteomic sample preparation strategy for identification and label-free quantification (LFQ) of ...
Thomas, Michna, Stefan, Tenzer
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Analysis of the Secondary Metabolism in Magnaporthe oryzae
2021Magnaporthe oryzae produces a number of secondary metabolites, some of which are thought to be responsible for the virulence of this fungus toward rice. Due to the importance of understanding plant-pathogen interactions, several of these metabolites have been investigated chemically and biosynthetically.
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Meiotic behavior of a supernumerary chromosome in Magnaporthe oryzae
Current Genetics, 2003A 1.2-Mb DNA band from an isolate of Magnaporthe oryzae was detected in a pulsed-field gel. A chromosomal entity corresponding to this band was observed at the mitotic metaphase stage. This minichromosome, carrying many transposable elements and two telomeres, was transmitted to ascosporic F(1) cultures in a non-Mendelian manner with frequent changes ...
I, Chuma +4 more
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