Results 71 to 80 of about 7,960,350 (301)

Dose‐dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction

FEBS Open Bio, EarlyView.
NMR metabolomics revealed concentration‐dependent metabolic perturbations in HepG2 cells exposed to H2O2. Rifampicin pretreatment enhanced metabolic competence, attenuated toxin‐induced alterations and produced metabolite profiles more consistent with human liver physiology, supporting the use of CYP450‐induced HepG2 models for improved in vitro ...
Maren Jinks, Garth L. Maker, Emily C. Davies, Berin A. Boughton, Samantha Lodge   +4 more
wiley   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Recent Progress in Electrical Generators for Oceanic Wave Energy Conversion

IEEE Access, 2020
Oceanic wave energy extraction through electrical generator is one of the most interesting topics in the field of power engineering. Almost all the existing relevant review paper focus on electrical generator with the working principle of electromagnetic
Abidur Rahman, Omar Farrok, Md. Rabiul Islam, Wei Xu   +3 more
doaj   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Dual Immobilization and Magnetic Manipulation of Magnetic Nanoparticles

, 2011
By suitably bio-functionalizing the surfaces, magnetic nanoparticles are able to bind specific biomolecules, and may serve as vectors for delivering bio-entities to target tissues.
YANG, SHIEH-YUEH;JIAN, ZHENG-FENG;HORNG, HERNG-ER;HONG, CHIN-YIH;YANG, HONG-CHANG;WU, CHAU-CHUNG, 楊謝樂;簡正峰;洪姮娥;洪振義;楊鴻昌;吳造中   +1 more
core   +1 more source

Removal of Mercury (II) by EDTA-Functionalized Magnetic CoFe₂O₄@SiO₂ Nanomaterial with Core-Shell Structure

Nanomaterials, 2019
In order to reduce the difficulty and risk of operation, decrease the preparation time and improve the adsorption performance of magnetic nano-silicon adsorbent with core-shell structure, a carboxylated CoFe2O4@SiO2 was prepared by EDTA-functionalized ...
Kai Xia, Yongfu Guo, Qijun Shao, Qu Zan, Renbi Bai   +4 more
doaj   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

In vitro generation of regulatory T cells: A challenging tool for studying immune aging in humans

FEBS Open Bio, EarlyView.
In vitro induction of human regulatory T cells using standard protocols generates suppressive, Foxp3‐expressing CD4+ T cells independently of TGF‐β supplementation and donor age. Regulatory T (Treg) cells play a key role in immune tolerance and homeostasis.
Lynn Muller, Jana Bleher, Birgit Weinberger, Lourdes Rocamora‐Reverte   +3 more
wiley   +1 more source

A near-infrared ratio fluorescent probe achieves mitochondrial hydrogen polysulfide imaging for monitoring ferroptosis in arthritis

Synthetic and Systems Biotechnology
Reactive sulfur (RSS) is a type of sulfur-containing molecule widely present in biological systems. Hydrogen polysulfide (H2Sn, n > 1), as a member of the active sulfur family, plays an indispensable role in many physiological and pathological processes.
Ting Cao, Ziwen Xiao, Wenhua Dong, Hong Ma, Deyan Gong, Zhefeng Fan   +5 more
doaj   +1 more source