Results 91 to 100 of about 10,645 (268)

Magnetic Tweezers: Micromanipulation and Force Measurement at the Molecular Level

, 2002
Cantilevers and optical tweezers are widely used for micromanipulating cells or biomolecules for measuring their mechanical properties. However, they do not allow easy rotary motion and can sometimes damage the handled material.
Gosse, Charlie, Croquette, Vincent, Charlie Gosse, Vincent Croquette   +3 more
core   +1 more source

AI‐Assisted Digital Single‐Molecule Activity Tracker for Decoupling Intrinsic Heterogeneity from Photo‐Oxidative Damage in High‐Photon‐Flux Enzymology

Advanced Science, EarlyView.
Employing a digital single‐molecule activity tracker (dSMAT), this research demonstrates that high‐photon‐flux irradiation drives progressive oxidative scarring in polymerases. Unlike simple thermal denaturation, real‐time kinetic tracking dynamically visualizes enzymes degrading into multiple impaired subpopulations.
Anran Zheng, Qi Yang, Jinze Li, Fuqiang Ma, Xuefeng Wang, Zhen Guo, Chuanyu Li, Dongshu Li, Jia Yao, Zhiqi Zhang, Wei Zhang, Lianqun Zhou   +11 more
wiley   +1 more source

Mechanochemistry Meets Catalysis: Metal Complexes for Greener Organic Transformations

Angewandte Chemie, EarlyView.
Mechanochemistry is redefining metal catalysis by controlling catalyst formulation, speciation, and deployment. This Review shows how milling, LAG, RAM, and TSE enable rapid metal‐complex assembly, distinctive catalytic manifolds, and scalable synthesis beyond solution chemistry.
Sourav Behera, Francesco Basoccu, Andrea Porcheddu   +2 more
wiley   +2 more sources

Homology sensing via non-linear amplification of sequence-dependent pausing by RecQ helicase

eLife, 2019
RecQ helicases promote genomic stability through their unique ability to suppress illegitimate recombination and resolve recombination intermediates. These DNA structure-specific activities of RecQ helicases are mediated by the helicase-and-RNAseD like C-
Yeonee Seol, Gábor M Harami, Mihály Kovács, Keir C Neuman   +3 more
doaj   +1 more source

Magnetic Tweezers for the Study of DNA Tracking Motors [PDF]

, 2010
Single-molecule manipulation methods have opened a new vista on the study of molecular motors. Here we describe the use of magnetic traps for the investigation of the mechanism of DNA based motors, in particular helicases and translocases.
Maria, Manosas, Adrien, Meglio, Michelle M, Spiering, Fangyuan, Ding, Stephen J, Benkovic, François-Xavier, Barre, Omar A, Saleh, Jean François, Allemand, David, Bensimon, Vincent, Croquette   +9 more
openaire   +2 more sources

Magnetic tweezers for captive disclination loops

, 2019
Disclination loops threaded on a polymeric fiber with planar axial anchoring are shown to be sensitive to magnetic fields. In particular, a homogeneous magnetic field oblique to the fiber drives translation of disclination loops along it. The velocity of
Maria Helena Godinho, Pawel Pieranski, Rui Cabeça, Pieranski, Pawel, Godinho, Maria Helena, Cabeça, Rui   +5 more
core   +1 more source

Flexible Thin‐Film Heater Composed of Laser‐Induced Graphene and Poly(D, L‐Lactic Acid) with Electro‐ and Photo‐Thermal Properties toward Cancer Thermotherapy

Advanced Electronic Materials, EarlyView.
This study introduces a thin‐film‐type heat‐generating device that uses laser induced graphene (LIG) on a poly(D, L‐lactic) acid film, which realizes both resistance heating by direct current application and photothermal conversion heating by near‐infrared light irradiation.
Masato Saito, Sota Harada, Ran Imai, Kon Son, Toshinori Fujie   +4 more
wiley   +1 more source

Coordination‐Driven Direct C─H Metalation of N‐Heterocycles With a Superbasic Co(II) Amide Co(TMP)2

Angewandte Chemie, EarlyView.
By enabling regioselectivities that are inaccessible with conventional bases, this study introduces a Co(II) amide platform for the deprotonative C─H metalation of sensitive N‐heterocycles. Subsequent interception of the resulting Co(II) intermediates with external oxidants affords a family of sterically congested, TMP‐substituted heterocycles via C─N ...
Na Jin, Manting Mu, Max García‐Melchor, Eva Hevia   +3 more
wiley   +2 more sources

Developing Magnetic Tweezers for Magnetic Material Manipulation

, 2015
Single-molecule research has stimulated the development of a wide range of technologies that are capable of manipulating very small structures and materials.
Liu, Yun
core  

Quantitative guidelines for force calibration through spectral analysis of magnetic tweezers data.

, 2010
Single-molecule techniques are powerful tools that can be used to study the kinetics and mechanics of a variety of enzymes and their complexes. Force spectroscopy, for example, can be used to control the force applied to a single molecule and thereby ...
Jacob W.J. Kerssemakers, Aartjan J.W. te Velthuis, Kerssemakers, Jacob W.J., te Velthuis, Aartjan J. W., te Velthuis, AJ, Nynke H. Dekker, Kerssemakers, Jacob W. J., Kerssemakers, JW, Lipfert, J, Dekker, NH, Lipfert, Jan, Jan Lipfert, te Velthuis, Aartjan J.W., Dekker, Nynke H.   +13 more
core   +1 more source