Results 11 to 20 of about 164,989 (120)

Purification of native histidine-rich protein 2 (nHRP2) from Plasmodium falciparum culture supernatant, infected RBCs, and parasite lysate

Malaria Journal, 2021
Background Despite the widespread use of histidine-rich protein 2 (HRP2)-based rapid diagnostic tests (RDTs), purified native HRP2 antigen is not standardly used in research applications or assessment of RDTs used in the field.
Balwan Singh, Jessica N. McCaffery, Amy Kong, Yong Ah, Scott Wilson, Sayan Chatterjee, Deepak Tomar, Michael Aidoo, Venkatachalam Udhayakumar, Eric Rogier   +9 more
doaj   +1 more source

Stability testing of dried Plasmodium falciparum positive quality control samples for malaria rapid diagnostic tests in Liberia and Benin

Malaria Journal, 2020
Background Malaria rapid diagnostic tests (RDTs) are largely responsible for the gains made in the proportion of malaria cases confirmed with a parasitological test. However, quality assurance programs to support their use remain a challenge.
Saliou Ramani, Henry T. Kohar, Oliver Pratt, Yves Eric Denon, Christie M. Reed, Peter Thomas, Suzanne E. Powell, Michael Aidoo   +7 more
doaj   +1 more source

Clearance dynamics of lactate dehydrogenase and aldolase following antimalarial treatment for Plasmodium falciparum infection

Parasites & Vectors, 2019
Background Lingering post-treatment parasite antigen in blood complicates malaria diagnosis through antigen detection. Characterization of antigen clearance dynamics is important for interpretation of positive antigen detection tests.
Mateusz M. Plucinski, Peter D. McElroy, Pedro Rafael Dimbu, Filomeno Fortes, Doug Nace, Eric S. Halsey, Eric Rogier   +6 more
doaj   +1 more source

Prevalence of K13 mutation and Day-3 positive parasitaemia in artemisinin-resistant malaria endemic area of Cambodia: a cross-sectional study

Malaria Journal, 2017
Background The presence of artemisinin-resistant malaria parasites was confirmed in western Cambodia in 2009. In 2013, mutations in the propeller domain of the kelch protein K13 was found to be associated with artemisinin resistance.
Soy Ty Kheang, Siv Sovannaroth, Sovann Ek, Say Chy, Phally Chhun, Sokkieng Mao, Sokomar Nguon, Dy Soley Lek, Didier Menard, Neeraj Kak   +9 more
doaj   +1 more source

Multiplex malaria antigen detection by bead-based assay and molecular confirmation by PCR shows no evidence of Pfhrp2 and Pfhrp3 deletion in Haiti

Malaria Journal, 2019
Background The Plasmodium falciparum parasite is the only human malaria that produces the histidine-rich protein 2 and 3 (HRP2/3) antigens. Currently, HRP2/3 are widely used in malaria rapid diagnostic tests (RDTs), but several global reports have ...
Camelia Herman, Curtis S. Huber, Sophie Jones, Laura Steinhardt, Mateusz M. Plucinski, Jean F. Lemoine, Michelle Chang, John W. Barnwell, Venkatachalam Udhayakumar, Eric Rogier   +9 more
doaj   +1 more source

Performance of antigen detection for HRP2-based malaria rapid diagnostic tests in community surveys: Tanzania, July–November 2017

Malaria Journal, 2022
Background Malaria rapid diagnostic tests (RDTs) based on the detection of the Plasmodium falciparum histidine-rich protein 2 (HRP2) antigen are widely used for detection of active infection with this parasite and are the only practical malaria ...
Eric Rogier, Catherine Bakari, Celine I. Mandara, Mercy G. Chiduo, Mateusz Plucinski, Douglas Nace, Nastassia Battle, Franky Chacky, Susan F. Rumisha, Fabrizio Molteni, Renata Mandike, Sigsbert Mkude, Ritha Njau, Ally Mohamed, Venkatachalam Udhayakumar, Deus S. Ishengoma   +15 more
doaj   +1 more source

Prevalence of molecular markers of artemisinin and lumefantrine resistance among patients with uncomplicated Plasmodium falciparum malaria in three provinces in Angola, 2015

Malaria Journal, 2018
Background Artemisinin-based combination therapy is the first-line anti-malarial treatment for uncomplicated Plasmodium falciparum infection in Angola.
Dragan Ljolje, Pedro Rafael Dimbu, Julia Kelley, Ira Goldman, Douglas Nace, Aleixo Macaia, Eric S. Halsey, Pascal Ringwald, Filomeno Fortes, Venkatachalam Udhayakumar, Eldin Talundzic, Naomi W. Lucchi, Mateusz M. Plucinski   +12 more
doaj   +1 more source

Efficacy and safety of artemether-lumefantrine and dihydroartemisinin-piperaquine for the treatment of uncomplicated Plasmodium falciparum malaria and prevalence of molecular markers associated with artemisinin and partner drug resistance in Uganda

Malaria Journal, 2021
Background In Uganda, artemether-lumefantrine (AL) is first-line therapy and dihydroartemisinin-piperaquine (DP) second-line therapy for the treatment of uncomplicated malaria.
Chris Ebong, Asadu Sserwanga, Jane Frances Namuganga, James Kapisi, Arthur Mpimbaza, Samuel Gonahasa, Victor Asua, Sam Gudoi, Ruth Kigozi, James Tibenderana, John Bosco Bwanika, Agaba Bosco, Denis Rubahika, Daniel Kyabayinze, Jimmy Opigo, Damian Rutazana, Gloria Sebikaari, Kassahun Belay, Mame Niang, Eric S. Halsey, Leah F. Moriarty, Naomi W. Lucchi, Samaly S. Svigel Souza, Sam L. Nsobya, Moses R. Kamya, Adoke Yeka   +25 more
doaj   +1 more source

Evaluating the quality of routinely reported data on malaria commodity stocks in Guinea, 2014–2016

Malaria Journal, 2018
Background Ensuring malaria commodity availability at health facilities is a cornerstone of malaria control. Since 2013, the Guinea National Malaria Control Programme has been routinely collecting data on stock levels of key malaria commodities through a
Yu Sun, Timothée Guilavogui, Alioune Camara, Mohamed Dioubaté, Babacar Deen Toure, Claude Bahati, Marie Paule Fargier, Jessica Butts, Patrick Condo, Abdoulaye Sarr, Mateusz M. Plucinski   +10 more
doaj   +1 more source

Community-based surveys for Plasmodium falciparum pfhrp2 and pfhrp3 gene deletions in selected regions of mainland Tanzania

Malaria Journal, 2020
Background Histidine-rich protein 2 (HRP2)-based malaria rapid diagnostic tests (RDTs) are effective and widely used for the detection of wild-type Plasmodium falciparum infections.
Catherine Bakari, Sophie Jones, Gireesh Subramaniam, Celine I. Mandara, Mercy G. Chiduo, Susan Rumisha, Frank Chacky, Fabrizio Molteni, Renata Mandike, Sigsbert Mkude, Ritha Njau, Camelia Herman, Douglas P. Nace, Ally Mohamed, Venkatachalam Udhayakumar, Caleb K. Kibet, Steven G. Nyanjom, Eric Rogier, Deus S. Ishengoma   +18 more
doaj   +1 more source