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Stabilization of halophilic malate dehydrogenase
Journal of Molecular Biology, 1989Malate dehydrogenase from the extreme halophile, Halobacterium marismortui, is stable only in highly concentrated solutions of certain salts. Previous work has established that its physiological environment is saturated in KCl; it remains soluble is saturated NaCl or KCl solutions; also it unfolds in solutions containing less than 2.5 M-NaCl or -KCl ...
Nina Borochov +4 more
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Malate dehydrogenase in leaf peroxisomes
Biochimica et Biophysica Acta (BBA) - Enzymology, 1969Abstract The technique of isopycnic centrifugation of leaf homogenates has allowed a separation of chloroplasts, mitochondria, and peroxisomes according to their respective densities. Chlorophyll, cytochrome c oxidase, and glycolate oxidase, respectively, were used as markers for these organeles. Malate dehydrogenase ( l -malate; NAD oxidoreductase,
R.K. Yamazaki, N. E. Tolbert
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Dissociation of mitochondrial malate dehydrogenase
Biochimica et Biophysica Acta (BBA) - Protein Structure and Molecular Enzymology, 1984The kinetics of the dissociation reaction under acidic conditions of the dimeric pig and chicken mitochondrial malate dehydrogenases (EC 1.1.1.37) have been studied. The dissociation of the pig enzyme is completely reversible. The pK for dissociation determined by light-scattering measurements agrees within experimental error with the pK value of 5.25 ...
Helmut Görisch +3 more
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Purification of Chlorella Malate Dehydrogenase
Preparative Biochemistry, 1972Abstract Our prior investigation of Chlorella malate dehydrogenase (MDH) revealed supernatant and particulate isoenzymes which were immunologically, chromatographically, and electro-phoretically distinct. By means of ammonium sulfate fractionation and column chromatography a crystalline preparation of the particulate isoenzyme, specific activity of ...
F. E. Cole, L. Naron
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Cooperativity in the mechanism of malate dehydrogenase
Biochemistry, 1993Cooperativity in the catalytic mechanism of porcine cytoplasmic malate dehydrogenase (sMDH) has been a point of ongoing discussion. Though previous investigations revealed little evidence of cooperativity, chemical modification studies reported by this laboratory demonstrate that binding of cofactor or cofactor plus substrate causes the enzyme's ...
Chris T. Zimmerle, Gerald M. Alter
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Evolution and Adaptation in Malate Dehydrogenases
The FASEB Journal, 2020What is the evolutionary relationship between structure, function and stability is a central concept in the molecular life sciences. Malate Dehydrogenase is an excellent model enzyme to ask questions about this concept since i) it exists throughout biology, playing a central role in energy metabolism and ii) has a number of isoforms including cytosolic,
Ellis Bell +2 more
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Starch-gel electrophoresis of malate dehydrogenase
Biochimica et Biophysica Acta (BBA) - Specialized Section on Enzymological Subjects, 1963Abstract Malate dehydrogenase preparations were subjected to electrophoresis on starch gel at pH 7.0. Purified preparations of mitochondrial malate dehydrogenase were shown to exist in up to six separable forms on the gel. The distribution pattern of these forms was not influenced by the age of the tissue of origin, the purification procedures used ...
Lawrence I. Grossman +2 more
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Two malate dehydrogenases in Methanobacterium thermoautotrophicum
Archives of Microbiology, 1998Methanobacterium thermoautotrophicum (strain Marburg) was found to contain two malate dehydrogenases, which were partially purified and characterized. One was specific for NAD+ and catalyzed the dehydrogenation of malate at approximately one-third of the rate of oxalacetate reduction, and the other could equally well use NAD+ and NADP+ as coenzyme and ...
Thompson, H. +3 more
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Properties of mitochondrial malate dehydrogenases
Biochimica et Biophysica Acta, 1962Abstract The malate dehydrogenases of the mitochondria of ox heart, rat liver and rabbit kidney and those extracted from acetone-dried powders of horse, pig and pigeon hearts have been purified and compared by a number of criteria. In substrate and coenzyme specificities, in the values for some Michaelis constants, and in their behaviour during ...
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Comparative Enzymology of Malate Dehydrogenases
Zeitschrift für Pflanzenphysiologie, 1975Summary 1. Total and specific activities of malate dehydrogenase, catalase, hexokinase, glucose-6-phosphate dehydrogenase and isocitrate dehydrogenase have been determined in extracts from seedlings of two hybrids and their parental lines of Zea mays. A correlation has been found between the genotype of seedlings and the levels of four enzymes during
V. Leskovac +3 more
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