Results 21 to 30 of about 7,790 (296)

Real‐Time Eco–AI, Electrophoresis‐Correlative Data‐Dependent Acquisition with AI‐Based Data Processing Broadens Access to Single‐Cell Mass Spectrometry Proteomics

Angewandte Chemie, EarlyView.
An affordable single‐cell proteomics workflow combines capillary electrophoresis, real‐time ion sorting, and AI‐aided spectral deconvolution to profile early Xenopus embryonic cells. Despite using a legacy Orbitrap, this strategy matches or exceeds the sensitivity of modern instruments, uncovering proteome dynamics at sub‐nanogram scale.
Bowen Shen, Fei Zhou, Peter Nemes
wiley   +2 more sources

Продуктивность и биохимический статус цыплят-бройлеров при использовании в их диете цитратов и малатов биометаллов [PDF]

, 2012
Приведены результаты влияния различных доз цитратов или малатов железа, марганца, цинка, меди и кобальта на рост и развитие цыплят-бройлеров, морфологический и биохимический состав крови, а так же химический состав их мышечной ...
Афанасьев, П. И., Горшков, Г. И., Кочеткова, Н. А., Шапошников, А. А., Шенцева, Е. А.   +4 more
core   +1 more source

Assessment of seismic vulnerability of historical defensive walls [PDF]

, 2015
This paper presents a study on the behaviour of the walls of the Rocca Roveresca of Senigallia in Italy built in the XIV century on the ruins of a former Roman defensive struc-ture. This is a peculiar example of a small fortress that had undergone in the
Dall'Asta, Andrea, Dezi, Luigino, Leoni, Graziano, Meschini, Alessandra, Petrucci, Enrica, Piattoni, Quintilio, Zona, Alessandro   +6 more
core   +1 more source

Antioxidant Properties of Pyrus communis and Pyrus pyrifolia Peel Extracts [PDF]

, 2020
With the rise of global health awareness by embracing a healthy lifestyle and natural product consumption, the search for natural antioxidant sources has invited more research performed especially in fruits as a whole product or in its components ...
Mardhatillah, Athina, Patricia, Vinda Maharani, Syaputri, Fauzia Ningrum, Tugon, Titian Daru Asmara   +3 more
core   +3 more sources

Short‐term actions of epigalocatechin‐3‐gallate in the liver: a mechanistic insight into hypoglycemic and potential toxic effects

FEBS Open Bio, EarlyView.
Epigallocatechin‐3‐gallate (EGCG) acutely inhibited gluconeogenesis and enhanced glycolysis, glycogenolysis, and fatty acid oxidation in perfused rat livers. Mechanistic assays revealed mitochondrial uncoupling, inhibition of pyruvate carboxylation and glucose‐6‐phosphatase, shift of NADH/NAD+ ratios toward oxidation, and loss of membrane integrity ...
Carla Indianara Bonetti, Bruna Lopes Correia, Francielle Cristina Nakamura Manicardi, Nairana Mithieli de Queiroz Eskuarek Melo, Vanesa de Oliveira Pateis, Jurandir Fernando Comar, Anacharis Babeto de Sá‐Nakanishi, Adelar Bracht, Lívia Bracht   +8 more
wiley   +1 more source

Structural and functional analysis of Bacillus sarcosine oxidase and its activity toward cyclic imino acids

FEBS Open Bio, EarlyView.
Sarcosine oxidase from Bacillus sp. (SoxB) recognizes l‐proline (l‐Pro), d‐proline (d‐Pro), and l‐thioproline (l‐Tpr) as minor substrates. In this study, we measured their absorption spectra, determined the crystal structures of their enzyme complexes, and performed site‐directed mutagenesis of key residues.
Yuqi Zhang, Yoshitaka Nakajima, Masae Kurobe, Tsutomu Nakamura, Tomoki Himiyama, Yoshiaki Nishiya   +5 more
wiley   +1 more source

eIF4E Enriched Extracellular Vesicles Induce Immunosuppressive Macrophages through HMGCR‐Mediated Metabolic Rewiring

Advanced Science, EarlyView.
Here, it is revealed that ovarian cancer cells release extracellular vesicles enriched with eIF4E, which alters protein production in macrophages. This leads to increased expression of 3‐hydroxy‐3‐methyl‐glutaryl‐coenzyme A reductase (HMGCR), boosting cholesterol synthesis and activating an immunosuppressive pathway through X‐box binding protein 1 and ...
Sonam Mittal, Minal Nenwani, Ishaque Pulikkal Kadamberi, Sudhir Kumar, Olamide Animasahun, Jasmine George, Shirng‐Wern Tsaih, Prachi Gupta, Mona Singh, Anjali Geethadevi, Chandrima Dey, Noah Meurs, Ajay Shankaran, Pradeep Chaluvally Raghavan, Deepak Nagrath, Sunila Pradeep   +15 more
wiley   +1 more source

A Genetically Encoded Biosensor for Characterizing Transport and Metabolism of Glutarate

Advanced Science, EarlyView.
Here, a genetically encoded glutarate biosensor, Glusor, is developed based on transcriptional regulator CsiR. Glusor can quantify glutarate concentrations with good accuracy and precision. Then, the role of KgtP and YnfM are identified and characterized by using Glusor. Glusor also allows glutarate spatiotemporal resolution in live cells, facilitating
Kaiyu Gao, Hui Zhang, Yidong Liu, Xianzhi Xu, Wei Liu, Zhaoqi Kang, Rong Xu, Shuang Hou, Ping Han, Chuanjuan Lü, Cuiqing Ma, Ping Xu, Chao Gao   +12 more
wiley   +1 more source

A Refined Adaptive Laboratory Evolution Strategy With Biosensor‐Assisted Selection Resolves the Tolerance–Efficiency Trade‐Off in Toxic Chemical Biosynthesis

Advanced Science, EarlyView.
This study presents a refined adaptive laboratory evolution (ALE) strategy to resolve the trade‐off between tolerance and biosynthetic efficiency in toxic chemical production. Combining in vivo mutagenesis, microdroplet‐based evolution, and biosensor‐assisted selection, this strategy rapidly yields robust “win‐win” phenotypes.
Yufei Zhang, Junhua Yun, Guoyan Zhang, Hossain M. Zabed, Yuehui Tian, Xinrui Tang, Jia Li, Xianghui Qi   +7 more
wiley   +1 more source

Xylose Metabolism Perturbation in Yarrowia lipolytica for Efficient Succinic Acid Bioproduction from Lignocellulosic Biomass

Advanced Science, EarlyView.
This study engineers Yarrowia lipolytica to produce succinic acid (SA) from lignocellulosic biomass. Adaptive evolution and multi‐omics analysis identify regulators that alleviate xylose metabolic disorder. Multi‐copy integration of xylose metabolic genes resolves redox imbalances from a futile fatty acid cycle, producing high‐titer SA from corn stover
Yutao Zhong, Changyu Shang, Jinhong Gu, Huilin Tao, Xuemei Lu, Jin Hou, Zhiyong Cui, Qingsheng Qi   +7 more
wiley   +1 more source