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长链非编码RNA CCAT1调控MAPK信号通路对于胶质瘤细胞的影响

Zhongguo shiyan zhenduanxue, 2020
目的探讨长链非编码RNA CCAT1表达对人胶质瘤细胞U251增殖、侵袭能力的影响及其作用机制。方法选取人胶质瘤细胞U251进行转染实验,分为空白组、sh-NC组和sh-CCAT1组;利用实时荧光定量qRT-PCR检测抑制效果;采用MMT、Transwell试验检测下调CCAT1对于胶质瘤细胞增殖、侵袭能力的影响;利用Western blot检测丝裂原活化蛋白激酶(MAPK)信号通路相关分子的表达变化。结果在转染48h后,sh-CCAT1组CCAT1的表达较NC组和sh-NC组显著降低 ...
王春辉, 孟繁凯, 费邵阳, 陈莫, 郑林杰   +4 more
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Review on Antioxidative Activities of L-Theanine(L-茶氨酸抗氧化作用的研究进展) [PDF]

Shipin kexue jishu xuebao, 2018
L-theanine is one kind of unique non-peptide amino acid, which is one of the main functional ingredients of tea. L-theanine has many physiological functions, such as antioxidative activity, regulating immune response, relaxing neural tension, and so on ...
LI Chengjian(李成舰), LUO Le(罗乐), HUANG Chunhua(黄春花)   +2 more
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Extraction and purification of Baizhu Shaoyao powder polysaccharides and their immunomodulatory effects on macrophages(白术芍药散多糖的分离纯化及其对巨噬细胞的免疫调节作用)

浙江大学学报. 农业与生命科学版
The present study aimed to extract and purify Baizhu Shaoyao powder polysaccharide (BSPP) and investigate its immunomodulatory effects on RAW264.7 macrophages.
ZHAO Ming(赵明), QIN Shiyue(秦诗越), ZHENG Sichun(郑斯纯), HOU Jiaxin(侯嘉欣), WANG Yushi(王雨诗), WANG Zihan(王子涵), MA Xiaodan(马晓丹), XU Wei(徐伟)   +7 more
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jck多囊肾小鼠肾囊泡上皮细胞MAPK信号通路上调

Zhongguo shiyan zhenduanxue, 2012
目的通过研究MAPK信号通路在多囊肾囊泡上皮细胞的调控,探讨多囊肾病的发病机制。方法以常染色体隐性遗传病jck多囊肾小鼠为模型,采用Western印迹法评价MAPK信号通路在jck多囊肾小鼠肾脏的表达和磷酸化调控。结果 jck多囊肾小鼠肾囊泡上皮细胞MAPK信号通路蛋白H-Ras、p-Raf、p-MEK、pERK、c-fos水平上调。结论 MAPK信号通路参与jck多囊肾小鼠肾囊泡的发生发展,提示MAPK通路可作为治疗多囊肾病的药物靶点。
阚秀芳, 赵丽晶, 李浩, 李丁洋, 邵晨, 谢爱国, 赵丽娟   +6 more
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肾病综合征氧化低密度脂蛋白脂质肾损伤的实验研究

Linchuang shenzangbing zazhi, 2014
目的 通过观察氧化低密度脂蛋白(oxidized low-density lipoprotein,ox-LDL)对系膜细胞(Mesangial Cells,MCs)分泌炎症介质功能的影响及MAPK信号通路和核因子-κB(nuclear factor kappa B,NF-κB)活性的改变,进一步阐明脂质在肾损伤中的作用机制。方法 利用ox-LDL诱导大鼠系膜细胞增殖,分别采用ELISA、real-time PCR、western blot技术检测MCs炎症介质、MAPK通路相关蛋白(p38、JNK ...
艾斯
doaj  

Abstracts of the 76th Annual Meeting of the Japanese Cancer Association; 2017 Sept 28‐30; Yokohama, Japan

, 2018
Cancer Science, Volume 109, Issue S1, Page 1-1325, January 2018.
wiley   +1 more source

硫化氢通过抑制ｐ３８ ＭＡＰＫ通路和坏死性凋亡的相互作用对抗高糖引起的Ｈ９Ｃ２ 心肌细胞损伤和炎症

Zhongshan Daxue xuebao. Yixue kexue ban, 2016
【目的】 探讨外源性硫化氢（Ｈ２Ｓ）能否通过抑制ｐ３８ 丝裂原激活蛋白激酶（ｐ３８ ＭＡＰＫ）通路和坏死性凋亡的相互作用对抗高糖引起的Ｈ９Ｃ２ 心肌细胞损伤和炎症。【方法】 采用细胞计数试剂盒８（ＣＣＫ－８）检测心肌细胞的存活率；ＥＬＩＳＡ法检测细胞培养液中白介素－１β（ＩＬ－１β）和肿瘤坏死因子－α（ＴＮＦ－α）的水平；蛋白质免疫印迹法（Ｗｅｓｔｅｒｎ Ｂｌｏｔ）测定ｐ３８ ＭＡＰＫ和ＲＩＰ３ 蛋白（反映坏死性凋亡的指标）的蛋白水平；双氯荧光素染色荧光显微镜照相测定细胞内活性氧（ＲＯＳ）水平 ...
梁伟杰
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Effects of Lipoteichoic Acid from Lactobacillus plantarum on Inflammatory Bowel Disease in Mice and Its Mechanism(植物乳杆菌脂磷壁酸对小鼠肠炎的作用及其机制) [PDF]

Shipin kexue jishu xuebao
To clarify the effect of lipoteichoic acid produced by Lactobacillus plantarum on enteritis, lipoteichoic acid from a probiotic Lactobacillus plantarum GSLP-7 was extracted, and gavaged to mice with enteritis.
ZHANG Luting(张露婷), LIU Juyuan(刘榉元), KONG Shuwen(孔淑文), CHEN Nuo(陈诺), YANG Bingyu(杨冰钰), XIA Huimei(夏慧妹), YANG Zhennai(杨贞耐), ZHANG Jian(张健)   +7 more
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Abstracts

Cancer Science, Volume 116, Issue S1, Page 1-1975, January 2025.
wiley   +2 more sources

#br# MAPK 及 MKP-1 在肾性高血压大鼠 心肌肥大过程中的变化

Zhongshan Daxue xuebao. Yixue kexue ban, 2000
【目的】研究肾性高血压大鼠( renal hypertensive rats, RHR)心肌肥大发生发展过程中心肌组织丝裂原活化蛋白 激酶(MAPK)活性、蛋白表达及丝裂原活化蛋白激酶磷酸酶(MKP-1)蛋白表达的变化以及血管紧张素Ⅱ( Ang Ⅱ) 1 型受体 (AT1)拮抗剂 TCV116 作用。【方法】①制作两肾一夹肾性高血压大鼠模型;②以左心室质量与体质量比值作为心肌肥大的 指标; ③以胶内 MBP 原位磷酸化法测定 MAPK 活性,以免疫印迹法检测 MKP-1 蛋白表达。【结果 ...
鲁　伟, 刘培庆, 陈健文, 潘敬运   +3 more
doaj