Results 161 to 170 of about 41,070 (215)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

FEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong, Yuhei Hosokawa, Lars Oliver Essen, Manuel Maestre‐Reyna   +3 more
wiley   +1 more source

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Materiales de construcción con materiales reciclados

Encuentro Internacional de Educación en Ingeniería, 2022
Los avances tecnológicos en los materiales de construcción benefician al ambiente porque propenden por alternativas sostenible. Por esta razón, es común encontrar, por ejemplo, cemento, bloques y metales que incorporan mejores propiedades mecánicas y a buen precio.
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Materiales Audiovisuales

Anuario Mexicano de Asuntos Globales
Missing Voices:Latin American Perspectives in International Relations [Spanish] International Studies Association (2024). Missing Voices: Latin American Perspectives in International Relations [Spanish]. [@intlstudiesassociation]. YouTube. https://youtu.be/xghq0-sr6N8?si=xR0aH-41eNfAhfNk ---------- War and International Politics | John Mearsheimer ...
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Endodontiske materialer - skyllemidler, sealere og obturerende materialer

Den norske tannlegeforenings Tidende, 2017
English summary Endodontic materials - irrigants, sealers, and obturating materials 248-56 The root canal filling concludes the classical triad in endodontic treatment: biomechanical instrumentation, disinfection and obturation. The purpose of the root canal filling is to prevent penetration of bacteria into
Håkon Valen, Gaute Floer Johnsen, Dag Ørstavik   +2 more
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Pulsiones materiales

2022
<p>Tal vez definir qué sea la materia o elaborar un único materialismo que cobije las complejidades de nuestro tiempo sea un trabajo destinado al fracaso. En lugar de ello este libro es un recorrido múltiple que sigue las trayectorias de la materia en espacios del pensamiento contemporáneo, artísticos, literarios y filosóficos. Simplificando, una
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Materialls for History

Camden Third Series, 1945
Being a Transcript of the Originall Commissions, Instructions, Orders, Debates, Letters, Messages, Military, Ecclesiasticall, Civill, in the Yeares 1658, 1659, 1660, 1661. Directed to the Lord Viscount Mordaunt and to other the Commissioners whose Names are inserted in the Plenepotentiary1.
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