Results 131 to 140 of about 23,598 (185)

Evolution‐guided yeast complementation reveals functional differences in human PSPH variants

FEBS Open Bio, EarlyView.
Ancient genomes can help guide which human genetic variants are tested experimentally. This study applies that idea to PSPH, a gene involved in serine biosynthesis, and uses high‐throughput yeast complementation to compare variant function. The findings reveal measurable differences among selected alleles and illustrate the value of evolution‐guided ...
Mauricio Campa‐Álvarez, Diana Ascencio, Miguel Vallebueno‐Estrada, Eduardo González‐Orozco, Christian Eduardo Martínez‐Guerrero, Rafael Montiel, Alexander DeLuna   +6 more
wiley   +1 more source

Defining Success and Failure In Prosthetic Joint Infections: A Meta-epidemiologic Study Toward A Core Outcome Set. [PDF]

Open Forum Infect Dis
Petri F, Alavi SMA, Ciubotariu CL, Passerini M, Borgonovo F, Matsuo T, Khawar H, Marson BA, Jagtiani A, Murad MH, Gori A, Tande AJ, Berbari EF.   +12 more
europepmc   +1 more source

Identifying and characterising a plant GH1 β‐glucosidase that exhibits hydrolytic activity on N‐linked glucopyranoside

FEBS Open Bio, EarlyView.
We report the first β‐glucosidase with demonstrated hydrolytic activity on an N‐linked glycopyranoside. The enzyme, native to maize, was biochemically characterised for this novel reaction, and structural modelling of the enzyme–substrate complex revealed several clues to the underlying reduced catalytic rate relative to its native O‐glycopyranoside ...
Hani Gharabli, Carlotta Chiesa, Maher Abou Hachem, Ditte Hededam Welner   +3 more
wiley   +1 more source

Luminescent core-isolated solvent-free liquids as a soft material platform for optical gas sensing. [PDF]

Chem Sci
Ishihara S, Ghosh A, Mori T, Chahal MK, Payne DT, Saeki A, Hyakutake T, Nakanishi T.   +7 more
europepmc   +1 more source

Structural studies and functional engineering of NanX: an anhydro‐sialic acid transporter from Escherichia coli

FEBS Open Bio, EarlyView.
Biophysical characterisation shows that NanX, a membrane transport protein from the major facilitator superfamily (MFS), forms both monomers and dimers after purification. AlphaFold modelling and substrate docking provide information on residues likely involved in substrate recognition for NanX and another MFS member, NanT.
Michael C. Newton‐Vesty, Kelsi R. Hall, Zachary D. Tillett, Liam S. Turk, James S. Davies, Michael J. Currie, Hamish G. Brown, Sepideh Valimehr, Peter D. Mace, Andrew E. Whitten, Santosh Panjikar, Eric Hanssen, Rachel A. North, Renwick C.J. Dobson   +13 more
wiley   +1 more source

Nitrogen Requirements in Healthy Adults: A Systematic Review and Meta-Analysis of Nitrogen Balance Studies. [PDF]

Nutrients
Suzuki D, Hayamizu K, Uno C, Hasegawa Y, Kuwahata M, Kido Y, Suzuki Y.   +6 more
europepmc   +1 more source

Adenosine triphosphate as a modulator of protein interactions and stability

FEBS Open Bio, EarlyView.
ATP is best known as the cell's energy currency, but it also shapes how proteins fold, interact, aggregate and form biomolecular condensates. This review explains the emerging physical principles behind these effects, including weak binding to charged protein regions, magnesium‐dependent behaviour and concentration‐dependent control of protein ...
Shuyuan Tan, Robin Curtis
wiley   +1 more source

Interventional Endoscopy for the Management of Post-Surgical Leaks and Fistulas: A Scoping Review. [PDF]

J Clin Med
Pessarelli T, Bergna IMB, Boemo C, De Monti A, La Milia M, Marfinati Hervoso C, Pagliarulo M, Piagnani A, Zago M, Amato A.   +9 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

Calculation of ultimate bearing capacity and analysis of bearing characteristics for pile group foundation to underlying offset cave. [PDF]

PLoS One
Zhao Y, Tao J, Li P, Yin L, Zhang Z, Yan H, Zhang W, Ma S, Liu J, Wang H.   +9 more
europepmc   +1 more source