Results 81 to 90 of about 282,015 (284)

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

open access: yesFEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz   +6 more
wiley   +1 more source

Pure simplicial complexes and well-covered graphs [PDF]

open access: yes, 2012
A graph $G$ is called well-covered if all maximal independent sets of vertices have the same cardinality. A simplicial complex $\Delta$ is called pure if all of its facets have the same cardinality.
Zaare-Nahandi, Rashid
core  

Combined 3D thinning and greedy algorithm to approximate realistic particles with corrected mechanical properties

open access: yes, 2019
The shape of irregular particles has significant influence on micro- and macro-scopic behavior of granular systems. This paper presents a combined 3D thinning and greedy set-covering algorithm to approximate realistic particles with a clump of ...
Yuan, Fei-Liang
core   +1 more source

KLK7 overexpression promotes an aggressive phenotype and facilitates peritoneal dissemination in colorectal cancer cells

open access: yesFEBS Open Bio, EarlyView.
KLK7, a tissue kallikrein‐related peptidase, is elevated in advanced colorectal cancer and associated with shorter survival. High KLK7 levels in ascites correlate with peritoneal metastasis. In mice, KLK7 overexpression increases metastasis. In vitro, KLK7 enhances cancer cell proliferation, migration, adhesion, and spheroid formation, driving ...
Yosr Z. Haffani   +6 more
wiley   +1 more source

Normal Covering Spaces with Maximal Bottom of Spectrum

open access: yesThe Journal of Geometric Analysis, 2023
AbstractWe study the property of spectral-tightness of Riemannian manifolds, which means that the bottom of the spectrum of the Laplacian separates the universal covering space from any other normal covering space of a Riemannian manifold. We prove that spectral-tightness of a closed Riemannian manifold is a topological property characterized by its ...
openaire   +2 more sources

Mycobacterial cell division arrest and smooth‐to‐rough envelope transition using CRISPRi‐mediated genetic repression systems

open access: yesFEBS Open Bio, EarlyView.
CRISPRI‐mediated gene silencing and phenotypic exploration in nontuberculous mycobacteria. In this Research Protocol, we describe approaches to control, monitor, and quantitatively assess CRISPRI‐mediated gene silencing in M. smegmatis and M. abscessus model organisms.
Vanessa Point   +7 more
wiley   +1 more source

Maximal Good Step Graph Methods for Reducing the Generation of the State Space

open access: yesIEEE Access, 2019
This paper proposes an effective method based on the two main partial order techniques which are persistent sets and covering step graph techniques, to deal with the state explosion problem. First, we introduce a new definition of sound steps, the firing
Hao Dou   +4 more
doaj   +1 more source

Tumor‐stromal crosstalk and macrophage enrichment are associated with chemotherapy response in bladder cancer

open access: yesFEBS Open Bio, EarlyView.
Chemoresistance in bladder cancer: Macrophage recruitment associated with CXCL1, CXCL5 and CXCL8 expression is characteristic of Gemcitabine/Cisplatin (Gem/Cis) Non‐Responder tumors (right side) while Responder tumors did not show substantial tumor‐stromal crosstalk (left side). All biological icons are attributed to Bioicons: carcinoma, cancerous‐cell‐
Sophie Leypold   +11 more
wiley   +1 more source

Polarization‐resolved femtosecond Vis/IR spectroscopy tailored for resolving weak signals in biological samples using minimal sample volume

open access: yesFEBS Open Bio, EarlyView.
Unique biological samples, such as site‐specific mutant proteins, are available only in limited quantities. Here, we present a polarization‐resolved transient infrared spectroscopy setup with referencing to improve signal‐to‐noise tailored towards tracing small signals. We provide an overview of characterizing the excitation conditions for polarization‐
Clark Zahn, Karsten Heyne
wiley   +1 more source

On maximal curves and unramified coverings

open access: yes, 2001
We discuss sufficient conditions for a given curve to be covered by a maximal curve with the covering being unramified; it turns out that the given curve itself will be also maximal. We relate our main result to the question of whether or not a maximal curve is covered by the Hermitian curve. We also provide examples illustrating the results.
Fuhrmann, Rainer   +2 more
openaire   +2 more sources

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