Results 1 to 10 of about 2,219 (151)

Multiple cross displacement amplification (MCDA) for rapid detection of toxigenic Clostridioides difficile as a potential point-of-care testing [PDF]

Microbiology Spectrum
Toxigenic Clostridioides difficile is the cause of C. difficile infection, highlighting the critical need for rapid and easy-to-use detection. In this study, a multiple cross displacement amplification (MCDA) assay was developed to detect toxigenic C ...
Hui Hu, Xiaomei Zhang, Mengting Cai, Xiaojun Song, Hongrui Cheng, Fangya Zhai, Yulei Tai, Zheng Xu, Shan Lin, Yu Chen, Yun Luo, Dazhi Jin   +11 more
doaj   +2 more sources

Development and Application of a Multiple Cross Displacement Amplification Combined With Nanoparticle-Based Lateral Flow Biosensor Assay to Detect Candida tropicalis

Frontiers in Microbiology, 2021
Candida tropicalis is an increasingly opportunistic pathogen that causes serious invasive candidiasis threatening a patient’s life. Traditional methods to detect C. tropicalis infection depends on time-consuming, culture-based gold-standard methods.
Yu Wang, Yu Wang, Xue Zhao, Jinzhi Cheng, Xiaomin Tang, Xu Chen, Honglan Yu, Shijun Li   +7 more
doaj   +1 more source

Sistema de avaliação de desempenho para apoio à gestão da coordenadoria dos processos licitatórios de um Hospital Universitário

Revista Ambiente Contábil, 2021
Objetivo: O objetivo da pesquisa é estruturar um modelo para apoiar a gestão da Coordenadoria de Processos Licitatórios do Hospital Universitário Professor Polydoro Ernani de São Thiago da Universidade Federal de Santa Catarina, visando construir, no seu
Samuel Souza de Araújo, Lucas dos Santos Matos, Sandra Rolim Ensslin   +2 more
doaj   +1 more source

A CRISPR-Cas12a-based platform for ultrasensitive rapid highly specific detection of Mycobacterium tuberculosis in clinical application

Frontiers in Cellular and Infection Microbiology, 2023
Tuberculosis, caused by Mycobacterium tuberculosis (MTB), is the second leading cause of death after COVID-19 pandemic. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based biosensing system to design a ...
Nan Jia, Chaohong Wang, Xiaming Liu, Xiaolan Huang, Fei Xiao, Jin Fu, Chunrong Sun, Zheng Xu, Guirong Wang, Juan Zhou, Yi Wang   +10 more
doaj   +1 more source

Real-time multiple cross displacement amplification assay for rapid and sensitive detection of Haemophilus influenzae

Frontiers in Cellular and Infection Microbiology, 2022
Haemophilus influenzae is an opportunistic pathogen usually causing bacteremia, meningitis, and pneumonia in children. Here, we developed a method based on multiple cross displacement amplification (MCDA) method and real-tme fluorescence technique for ...
Chunrong Sun, Nan Jia, Xiaolan Huang, Fei Xiao, Juan Zhou, Yu Zhang, Jin Fu, Zheng Xu, Dong Qu, Xiaodai Cui, Yi Wang   +10 more
doaj   +1 more source

Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use

Frontiers in Cellular and Infection Microbiology, 2022
Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection (STI) and remains a major public health challenge, especially in less-developed regions.
Xu Chen, Xu Chen, Wei Yuan, Qingxue Zhou, Yan Tan, Ronghua Wang, Shilei Dong   +6 more
doaj   +1 more source

A CRISPR-Cas12a—Based platform for ultrasensitive, rapid, and highly specific detection of Mycoplasma pneumonia in clinical application

Frontiers in Bioengineering and Biotechnology, 2023
Mycoplasma pneumoniae (MP), which is responsible for a majority of community-acquired pneumonia (CAP) in children, has been largely underestimated. Here, we coupled multiple cross displacement amplification (MCDA) technique with CRISPR-Cas12a-based ...
Nan Jia, Juan Zhou, Fei Xiao, Baoying Zheng, Xiaolan Huang, Chunrong Sun, Jin Fu, Zheng Xu, Min Chen, Yi Wang   +9 more
doaj   +1 more source

Development of performance indicators for Occupational Health and Safety: a constructivist multicriteria approach for PPE

Production, 2021
Paper aims This study aims to develop indicators, using the Multi-Criteria Decision Aid - Constructivist methodology (MCDA-C), to assess the occupational prevention activities in a psychiatric hospital.
Carmen Elena Martínez Riascos, Sandra Rolim Ensslin, Eugenio Andrés Díaz Merino   +2 more
doaj   +1 more source

Instrumento para identificação das necessidades do consumidor no processo de desenvolvimento do design: um estudo ilustrado com o projeto de um automóvel Instrument for identifying consumer needs in the design development process: the case of an automobile design

Gestão & Produção, 2011
A inovação é hoje uma das principais prioridades para o desenvolvimento de um novo produto e nela o design emerge com destaque, principalmente quando consegue encapsular as necessidades e preferências do usuário.
Cleci Grzebieluckas, Marcos Albuquerque Buson, Shirley Gomes Queiroz, Leonardo Ensslin, Sandra Ensslin, Elton Nickel, Alceu Junior Balbim   +6 more
doaj   +1 more source

Rapid Detection of Aspergillus fumigatus Using Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Lateral Flow

Frontiers in Cellular and Infection Microbiology, 2021
Aspergillus fumigatus is an opportunistic, ubiquitous, saprophytic mold which can cause infection in the lungs, nose, eyes, brain, and bones in humans, especially in immunocompromised patients. However, it is difficult to diagnose A.
Luxi Jiang, Xiaomeng Li, Xiaomeng Li, Rumeng Gu, Rumeng Gu, Deguang Mu   +5 more
doaj   +1 more source