Results 21 to 30 of about 180 (87)

Ultrasensitive and highly specific detection of the Brucella genus and B. melitensis by CRISPR/Cas12b‐multiple cross displacement amplification technique [PDF]

Journal of Clinical Microbiology
Brucellosis is caused by members of the Brucella spp. and remains one of the world’s major zoonotic diseases. Brucella melitensis (B. melitensis) as the most contagious Brucella species cannot be ignored as an essential source of infection for ...
Sha Mao, Xinggui Yang, Yue Wang, Fengming Chen, Hai Jiang, Yi Wang, Yingqian Kang, Shijun Li   +7 more
doaj   +2 more sources

Beyond Serology: A Meta-Analysis of Advancements in Molecular Detection of Brucella spp. in Seronegative Animals and Biological Samples. [PDF]

Vet Med Sci
Molecular diagnostics, such as PCR and biosensor‐based techniques, offer high sensitivity in detecting Brucella spp. in seronegative animals, overcoming the limitations of traditional serological methods. The pooled detection rate of 35.08% suggests a substantial number of hidden carriers.
Islam MS, Habib MA, Tonu NS, Haque MS, Rahman MM.   +4 more
europepmc   +2 more sources

Research on Product Innovation Intention Analysis System Model Based on Computational Thinking

Mathematical Problems in Engineering, Volume 2022, Issue 1, 2022., 2022
The starting point of this study is to improve the realistic problem of high failure rate of product innovation and improve the success rate of product innovation. First, starting from the relationship between user demand and innovation, this study constructs a product innovation‐demand screening system model from a macro perspective and describes the ...
Shuo Zhang, Hua Zou, Zhicheng Qiao, Jian Sun, Yan Xu, Luca Silvestri   +5 more
wiley   +1 more source

Rapid Detection of Aspergillus fumigatus Using Multiple Cross Displacement Amplification Combined With Nanoparticles-Based Lateral Flow

Frontiers in Cellular and Infection Microbiology, 2021
Aspergillus fumigatus is an opportunistic, ubiquitous, saprophytic mold which can cause infection in the lungs, nose, eyes, brain, and bones in humans, especially in immunocompromised patients. However, it is difficult to diagnose A.
Luxi Jiang, Xiaomeng Li, Xiaomeng Li, Rumeng Gu, Rumeng Gu, Deguang Mu   +5 more
doaj   +1 more source

Additional file 1 of Multiple Cross Displacement Amplification Coupled with Lateral Flow Biosensor (MCDA-LFB) for rapid detection of Legionella pneumophila

, 2022
Additional file 1.
Jiang, Luxi, Gu, Rumeng, Li, Xiaomeng, Song, Meijun, Huang, Xiaojun, Mu, Deguang   +5 more
openaire   +1 more source

Highly Sensitive and Rapid Identification of Streptococcus agalactiae Based on Multiple Cross Displacement Amplification Coupled With Lateral Flow Biosensor Assay

Frontiers in Microbiology, 2020
Streptococccus agalactiae (S. agalactiae) is an important neonatal pathogen that is associated with mortality and morbidity. Therefore, we developed a rapid, accurate, and sensitive method based on multiple cross displacement amplification (MCDA) for the
Xueqin Cheng, Xueqin Cheng, Zhiqian Dou, Jing Yang, Yulong Gu, Dexi Liu, Ling Xie, Tao Ren, Yan Liu, Zhifang Yu, Yijun Tang, Meifang Wang   +11 more
doaj   +1 more source

Establishment and application of a multiple cross displacement amplification combined with nanoparticles-based biosensor method for the detection of Bordetella pertussis

BMC Microbiology, 2020
Background Bordetella pertussis is the causative agent of pertussis, a respiratory tract infectious disease. Efficient techniques for detection of B. pertussis isolates are important for clinical diagnosis. Multiple cross displacement amplification (MCDA)
Shijun Li, Chunting Liu, Ying Liu, Qing Ma, Yue Wang, Yi Wang   +5 more
doaj   +1 more source

Rapid Detection of Brucella spp. and Elimination of Carryover Using Multiple Cross Displacement Amplification Coupled With Nanoparticles-Based Lateral Flow Biosensor

Frontiers in Cellular and Infection Microbiology, 2019
Brucella spp.is capable of causing disease in a range of animal hosts, and human brucellosis is regarded as a life-threating disease. A novel isothermal amplification technique, termed multiple cross displacement amplification (MCDA), was employed for ...
Shijun Li, Ying Liu, Yue Wang, Ming Wang, Chunting Liu, Yi Wang   +5 more
doaj   +1 more source

Multiple Cross Displacement Amplification Linked with Nanoparticles-Based Lateral Flow Biosensor in Screening of Hepatitis B Virus in Clinical Application

Infection and Drug Resistance, 2021
Xu Chen,1,2,* Qingxue Zhou,3,* Shilei Dong,4 Shuoshi Wang,2 Rui Liu,2 Xueli Wu,2 Shijun Li5 1The Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550003, People’s Republic of China; 2Central Laboratory ...
Chen X, Zhou Q, Dong S, Wang S, Liu R, Wu X, Li S   +6 more
doaj  

Establishment and Application of a Multiple Cross Displacement Amplification Coupled With Nanoparticle-Based Lateral Flow Biosensor Assay for Detection of Mycoplasma pneumoniae

Frontiers in Cellular and Infection Microbiology, 2019
Mycoplasma pneumoniae (M. pneumoniae) is responsible for pneumonia, and is a causative agent of other respiratory tract infections (e.g., bronchiolitis and tracheobronchitis). Herein, we established and applied a multiple cross displacement amplification
Yacui Wang, Yi Wang, Shuting Quan, Weiwei Jiao, Jieqiong Li, Lin Sun, Yonghong Wang, Xue Qi, Xingyun Wang, Adong Shen   +9 more
doaj   +1 more source