Sensitive and visual identification of Chlamydia trachomatis using multiple cross displacement amplification integrated with a gold nanoparticle-based lateral flow biosensor for point-of-care use [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Chlamydia trachomatis is the leading cause of bacterial sexually transmitted infection (STI) and remains a major public health challenge, especially in less-developed regions.
Xu Chen +6 more
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Real-time multiple cross displacement amplification assay for rapid and sensitive detection of Haemophilus influenzae [PDF]
Frontiers in Cellular and Infection Microbiology, 2022 Haemophilus influenzae is an opportunistic pathogen usually causing bacteremia, meningitis, and pneumonia in children. Here, we developed a method based on multiple cross displacement amplification (MCDA) method and real-tme fluorescence technique for ...
Chunrong Sun +10 more
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Multiple Cross Displacement Amplification Linked with Nanoparticles-Based Lateral Flow Biosensor in Screening of Hepatitis B Virus in Clinical Application [PDF]
Infection and Drug Resistance, 2021 Xu Chen,1,2,* Qingxue Zhou,3,* Shilei Dong,4 Shuoshi Wang,2 Rui Liu,2 Xueli Wu,2 Shijun Li5 1The Second Clinical College, Guizhou University of Traditional Chinese Medicine, Guiyang, Guizhou, 550003, People’s Republic of China; 2Central Laboratory ...
Chen X +6 more
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Development of a multiple cross displacement amplification combined with nanoparticles-based biosensor assay for rapid and sensitive detection of Streptococcus pyogenes [PDF]
BMC MicrobiologyBackground S. pyogenes, is a primary pathogen that leads to pharyngitis and can also trigger severe conditions like necrotizing fasciitis and streptococcal toxic shock syndrome (STSS), often resulting in high mortality rates.
Zhiqian Dou +3 more
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Frontiers in Microbiology, 2020 Streptococccus agalactiae (S. agalactiae) is an important neonatal pathogen that is associated with mortality and morbidity. Therefore, we developed a rapid, accurate, and sensitive method based on multiple cross displacement amplification (MCDA) for the
Yijun Tang, Meifang Wang, Tao Ren
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The new gold rush in clinical diagnostics: from standard laboratory assays to fast and accurate point-of-care (POC) methods [PDF]
Microbiology SpectrumIntegrating two point-of-care (POC)-amenable technologies, namely, multiple cross displacement amplification (MCDA) and gold nanoparticle-based lateral flow biosensors (AuNPs-LFB), has demonstrated excellent detection of hepatitis B virus (HBV) and ...
Hui-Chen Foreman
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Ultrasensitive and highly specific detection of the Brucella genus and B. melitensis by CRISPR/Cas12b‐multiple cross displacement amplification technique [PDF]
Journal of Clinical MicrobiologyBrucellosis is caused by members of the Brucella spp. and remains one of the world’s major zoonotic diseases. Brucella melitensis (B. melitensis) as the most contagious Brucella species cannot be ignored as an essential source of infection for ...
Sha Mao +7 more
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Sensors and Actuators ReportsHuman adenoviruses (HAdV), particularly serotypes 3 and 7 (HAdV-3 and HAdV-7), are significant respiratory pathogens that contribute to high morbidity rates and severe pneumonia in infants and children.
Linglong Wan +10 more
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Frontiers in Cellular and Infection Microbiology, 2019 Mycoplasma pneumoniae (M. pneumoniae) is responsible for pneumonia, and is a causative agent of other respiratory tract infections (e.g., bronchiolitis and tracheobronchitis). Herein, we established and applied a multiple cross displacement amplification
Yi Wang, Lin Sun
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