Results 171 to 180 of about 88,764,951 (299)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

open access: yesFEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli   +8 more
wiley   +1 more source

Using Restricted Mean Time Lost to Evaluate the Prognostic Effects on Locally Advanced Breast Cancer Considering Competing Risks [PDF]

open access: yes
Zhaojin Li,1 Di Liu,2 Yawen Hou,3 Zheng Chen1,4 1Department of Biostatistics, School of Public Health (Guangdong Provincial Key Laboratory of Tropical Disease Research), Southern Medical University, Guangzhou, People’s Republic of China; 2Department of ...
Li Z, Chen Z, Liu D, Hou Y
core  

The Impact Of AI-based Root Cause Analysis On Reducing Mean Time To Repair (MTTR)

open access: yes
Artificial Intelligence (AI)-based Root Cause Analysis (RCA) has emerged as a transformative approach in industrial and IT operations, profoundly impacting the efficiency and speed of problem resolution. Traditionally, diagnosing and rectifying issues on complex systems required manual investigation, often leading to prolonged mean time to repair (MTTR)
openaire   +2 more sources

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

EVIDENCE-BASED REVIEWS & RECOMMENDATIONS FROM THE BRAZILIAN SOCIETY OF SHOULDER AND ELBOW SURGERY: EARLY VERSUS LATE REPAIR FOR TRAUMATIC ROTATOR CUFF TEARS. [PDF]

open access: yesActa Ortop Bras
Lara PHS   +7 more
europepmc   +1 more source

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