Results 191 to 200 of about 166,095,516 (255)

SPHINX31 acts as a SRPK1 inhibitor targeting the ATR/DNA‐PKcs/CHK1 replicative checkpoint to inhibit cell growth in non‐small cell lung cancer

Molecular Oncology, EarlyView.
The kinase SRPK1 directly interacts with the protein TOPBP1 and regulates the pre‐mRNA splicing of WIZ thereby contributing to the activation of the ATR/CHK1 replicative checkpoint in response to replicative stress. This allows cancer cells' genomic stability and survival.
Amani Shreim, Aurelie Genoux, Nadiia Zubchuk, Haoyang Zheng, Perla Salameh, Theo Ziegelmeyer, Fabien Dalonneau, Margot Rouchette, Christiane Oddou, Simona Miron, Helene Polveche, Adiilah Mamode Cassim, Carmen Garrido, Eleni Nikolakaki, Didier Auboeuf, Tao Jia, Sophie Zinn‐Justin, Beatrice Eymin   +17 more
wiley   +1 more source

Automated FRAP microscopy for high‐throughput analysis of protein dynamics in chromatin organization and transcription

FEBS Open Bio, EarlyView.
RoboMic is an automated confocal microscopy pipeline for high‐throughput functional imaging in living cells. Demonstrated with fluorescence recovery after photobleaching (FRAP), it integrates AI‐driven nuclear segmentation, ROI selection, bleaching, and analysis.
Selçuk Yavuz, Bart Geverts, Johan A. Slotman, Andrea Sacchetti, Stefan Prekovic, Martin E. van Royen, Adriaan B. Houtsmuller   +6 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

FEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee, Giulia Zanetti, Bram van den Broek, Kees Jalink   +3 more
wiley   +1 more source

Bacterial Expression of the Calmodulin-Binding Region of Smooth Muscle Myosin Light Chain Kinase

, 1985
Guerriero, V, Russo, M A, RUSSO, MARIO ANTONIO, Means, A R, Means, A. R.   +4 more
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Expressiveness, meanings and machines

Computability, 2018
There is considerable interest in constructing succinct programs and much debate over which languages are most expressive. However, such debates tend to revolve around syntactic notions of program size and language constructs, without considering the complex interrelations of syntax, semantics, and implementation.
Joe Davidson, Greg Michaelson
openaire   +2 more sources

Meaning and Use of Indefinite Expressions

Journal of Logic, Language and Information, 2002
Sentences containing an indefinite expression like `he' (pronoun) or `a man' (indefinite noun phrase) are said to express open propositions, i.e. propositions that display gaps to be filled. The author presents a unified and compositional analysis of those expressions in first-order logic that is based on the notions of content, update and support. For
openaire   +4 more sources

Expressive Meanings and Expressive Commitments. A Case of Meaning as Use

2019
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openaire   +2 more sources

Shared means and meanings in vocal expression of man and macaque

Logopedics Phoniatrics Vocology, 2003
Vocalisations of six Macaca arctoides that were categorised according to their social context and judgements of naive human listeners as expressions of plea/submission, anger, fear, dominance, contentment and emotional neutrality, were compared with vowel samples extracted from simulations of emotional-motivational connotations by the Finnish name ...
Lea, Leinonen, Maija-Liisa, Laakso, Synnöve, Carlson, Ilkka, Linnankoski   +3 more
openaire   +2 more sources