Results 201 to 210 of about 544,111 (317)

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Disaster Health Literacy - development and validation of a short measurement instrument in German to supplement the HLS₁₉ instruments. [PDF]

Front Public Health
Ewers M, Beckert J, Griese L, Köhler M, Prasser A, Singh H, Schaeffer D.   +6 more
europepmc   +1 more source

Weak Data: The Social Biography of a Measurement Instrument and How It Failed to Ensure Accountability in Home Care. [PDF]

Med Anthropol Q, 2020
Hoeyer K, Bødker M.
europepmc   +1 more source

Establishing an assay to evaluate d‐amino acid oxidase enzyme kinetics and inhibition using WST‐8 redox dye

FEBS Open Bio, EarlyView.
This study investigated a novel WST‐8‐based assay for evaluating d‐Amino acid oxidase (DAO) inhibitors. We confirmed its effectiveness using known inhibitors and found that uremic toxins possess relatively weak inhibitory activity compared to existing drugs.
Kahoko Miyake, Yuki Enoki, Yuka Nakazawa, Kazuaki Taguchi, Kazuaki Matsumoto   +4 more
wiley   +1 more source

Development and validation of a measurement instrument for student assessment of quality physical education in Chinese secondary schools. [PDF]

PLoS One
Qin L, Ho WKY, Khoo S.
europepmc   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source

Perspective integration capability: A valid and reliable measurement instrument for assessing knowledge integration readiness in interdisciplinary collaborations. [PDF]

J Clin Transl Sci
Salazar Campo M, Lant TK.
europepmc   +1 more source

Screening and epitope characterization of Nidogen‐2‐specific nanobodies

FEBS Open Bio, EarlyView.
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen, Qianqian Cui, Zhongyun Lan, Yingjun Wang, Shuaiying Zhao, Wenxuan Feng, Yunfeng Liu, Qiting Huang, Dongna Zhang, Jianfeng Xu   +9 more
wiley   +1 more source

Capability of measurement instrument

, 2013
Zdolność pomiarowa jest wskaźnikiem umożliwiającym ocenę jakości metrologicznej przyrządu. Miarą zdolności pomiarowej przyrządu jest niepewność rozszerzona obliczana po wykonaniu pomiaru na wzorcu pomiarowym.
Fotowicz, P.
core  

Pulmonary function in patients with adolescent idiopathic scoliosis: an explorative study of a wearable smart shirt as a measurement instrument. [PDF]

Spine Deform
Te Hennepe N, Steegh VLJM, Pouw MH, Roukema J, De Kleuver M, Van Hooff ML.   +5 more
europepmc   +1 more source