Results 61 to 70 of about 87,338 (265)

Interpreting the effects of DNA polymerase variants at the structural level

Molecular Oncology, EarlyView.
Using MAVISp and molecular dynamics simulations, we analyzed over 60 000 missense variants in POLE and POLD1 from ClinVar, COSMIC, cBioPortal, and saturation mutagenesis. Identified mechanistic indicators, including stability, binding, and long‐range, enable structural interpretation, providing ACMG‐like evidence for possible reclassification of VUS ...
Matteo Arnaudi, Karolina Krzesińska, Ludovica Beltrame, Pablo Sánchez‐Izquierdo Besora, Matteo Tiberti, Mef Nilbert, Anna Rohlin, Elena Papaleo   +7 more
wiley   +1 more source

The purposes of measurement uncertainty

AIP Conference Proceedings
It is now more than 40 years since the BIPM outlined the current harmonised approach to the expression of uncertainty in measurement, and 30 years since the publication of the Guide to the Expression of Uncertainty in Measurement. Despite several decades of use and application to a wide variety of measurements, there remains a lack of clarity about the
White, Rod, Hall, Blair, Saunders, Peter
openaire   +2 more sources

Finding novel vulnerabilities of hypomorphic BRCA1 alleles

Molecular Oncology, EarlyView.
Synthetic lethality screens performed to identify novel vulnerabilities often model complete gene loss, thereby overlooking patient‐derived hypomorphic mutations. In this study, we have performed genome‐wide CRISPR screens on BRCA1 hypomorphic mutations, showing BRCA1I26A behaves like wild‐type, while BRCA1R1699Q mimics deficiency. Furthermore, we have
Anne Schreuder, Klaas de Lint, Hồ Mỹ Phúc Võ, Mariana M. Góis, Rosalie A. Kampen, Marta San Martin Alonso, Ilse Nootenboom, Veronica Garzero, Tiemen J. Wendel, Rob M. F. Wolthuis, Sylvie M. Noordermeer   +10 more
wiley   +1 more source

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

FEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei, Fredrik Orädd, Konstantinos Magkakis, Magnus Andersson   +3 more
wiley   +1 more source

A method for evaluation of uncertainties of noise parameter measurement

Journal of Telecommunications and Information Technology, 2002
The assessment of uncertainties of a two-port noise parameters measurement, presented in the paper, relies on modeling of sources of errors and an investigation of propagation of the errors through a measurement system.
Marek Schmidt-Szałowski, Wojciech Wiatr
doaj   +1 more source

Uncertainties in successive measurements

Physical Review A, 2013
Improvements in the prose (thanks to the referee). Version to appear in Phys. Rev. A.
Distler, Jacques, Paban, Sonia
openaire   +3 more sources

Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies

FEBS Open Bio, EarlyView.
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli, Mohammad Nour Alsamsam, Ugnė Bagdonaitė, Van Truc Vu, Chun‐Jen Huang, Polina Kuzhir, Mindaugas Zaremba, Aurimas Kopūstas, Marijonas Tutkus   +8 more
wiley   +1 more source

Measurement Theory, Nomological Machine And Measurement Uncertainties (In Classical Physics)

Studia Philosophica Estonica, 2012
Measurement is said to be the basis of exact sciences as the process of assigning numbers to matter (things or their attributes), thus making it possible to apply the mathematically formulated laws of nature to the empirical world.
Ave Mets
doaj  

Uncertainty Propagation in GaN HEMT Small-Signal Models for Millimeter-Wave Circuit Design Applications

IEEE Journal of Microwaves
This paper investigates the impact of S-parameter measurement uncertainties on broadband modeling and circuit design using gallium nitride (GaN) high electron mobility transistors (HEMTs) from 0.5–110 GHz.
Jason Shell, Edward Gebara, Nicholas C. Miller   +2 more
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

FEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill, Yelyzaveta Pulnova, Elke De Zitter, Helen M. Ginn, Briony A. Yorke   +4 more
wiley   +1 more source