Results 141 to 150 of about 13,212 (177)

Assessment of oxacillin salt agar for detection of MRSA identified by presence of the mecA gene

Journal of Hospital Infection, 1993
To assess the screening method for detection of methicillin-resistant Staphylococcus aureus recommended by Thornsberry and McDougal J. Clin Microbiol 1983; 18: 1084-1901, the growth of S. aureus and Staphylococcus epidermidis strains on Mueller Hinton agar containing 4% NaCl and 6 mg l-1 oxacillin, after 24 h incubation at 35 degrees C, was ...
M Kizaki
exaly   +3 more sources

Surveillance of variant mecA gene and analysis of efflux pump in mecA negative MRSA infections

Pakistan Journal of Pharmaceutical Sciences, 2017
It has been known from the medical records of Kancheepuram district hospitals that there is a prevalence of Methicillin resistant Staphylococcus aureus strain. Now the recent epidemiology has shown the presence of Methicillin Resistant Staphylococcus aureus even in school going children community individuals. In view of the above situation, Methicillin
openaire   +1 more source

The Molecular Detection of mecA Genes of Staphylococcus Aureus

Annals of Advanced Biomedical Sciences, 2019
Antibiotic resistance is common among pathogenic bacteria associated with community acquired and nosocomial infections. Methicillin-resistant Staphylococcus aureus (MRSA) infections have become a global health problem particularly in hospital setup causing simple skin infections to life threatening infections. The present study aimed to investigate the
openaire   +1 more source

Rapid identification of bacteria, mecA and van genes from blood cultures

Pathologie Biologie, 2007
The Genotype technology, a quick molecular genetic assay based on DNA multiplex amplification with biotinylated primers followed by hybridization to membrane bound probes, complies with the requirements for a fast diagnosis of sepsis. We evaluated the new Genotype BC Gram-negative and Gram-positive test kits (Hain Life Science, Germany) which ...
M-F, Prère, O, Baron, O, Fayet
openaire   +2 more sources

Surveillance of variant mecA gene and analysis of efflux pump in mecA negative MRSA infections.

Pakistan journal of pharmaceutical sciences, 2019
It has been known from the medical records of Kancheepuram district hospitals that there is a prevalence of Methicillin resistant Staphylococcus aureus strain. Now the recent epidemiology has shown the presence of Methicillin Resistant Staphylococcus aureus even in school going children community individuals. In view of the above situation, Methicillin
Karthikeyan, Vijayan, Sulochana, Somasundaram, Catherine, Leo, Jayaprakash, Kanagaraj   +3 more
openaire   +1 more source

The Characterization of mecA Gene and SCCmec Typing in Clinical Samples of MRSA

Science Letters, 2019
MRSA is a major pathogen worldwide and its infections are associated with increased morbidity and mortality, in comparison with other S. aureus infections. The study was designed to characterize the mecA gene and staphylococcal cassette chromosome (SCCmec) in methicillin resistance S. aureus (MRSA). A total of 20 presumptive S.
Aziyah Abdul Aziz, Ali Jawad
openaire   +1 more source

[MRSA--detection of mecA and its regulatory genes].

Rinsho byori. The Japanese journal of clinical pathology, 1994
Methicillin resistance in S.aureus and S.epidermidis strains is primarily due to production of a new penicillin-binding protein PBP2' with extremely low binding affinity to most beta-lactam antibiotics. The structural gene for PBP2', mecA, is detectable in clinical specimens by using the polymerase chain reaction (PCR).
S, Kagawa, K, Yamashita, A, Matsuoka
openaire   +1 more source

[Combination PCR of mecA, femA genes for detection of MRSA].

Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2004
To make a comparison of PCR assay, Agar Dilution and Disk Diffusion as for detecting Staphylococcal mecA, femA genes.A total of 178 strains of Staphylococci were isolated from three large scale hospitals in Chengdu. Disk Diffusion and staphylococcal mecA, femA gene PCR assay for detecting MRSA were compared with Agar Dilution.The sensitivity and ...
Zhihong, Chen, Xinjian, Fan, Xiaoju, Lü
openaire   +1 more source

An ultrasensitive electrochemical biosensor for the detection of mecA gene in methicillin-resistant Staphylococcus aureus

Biosensors and Bioelectronics, 2018
Electrochemical DNA biosensor has unique advantages for on-site pathogenic microorganism detection, yet the detection of long DNA towards genome DNA (gDNA) analysis remains challenge. In this work, we report a novel electrochemical biosensor for the ultrasensitive analysis of mecA DNA on methicillin-resistant Staphylococcus aureus (MRSA) genome, using ...
Li, Xu, Wen, Liang, Yanli, Wen, Lele, Wang, Xue, Yang, Shuzhen, Ren, Nengqin, Jia, Xiaolei, Zuo, Gang, Liu   +8 more
openaire   +2 more sources