Results 121 to 130 of about 2,563,992 (349)

Calpain small subunit homodimerization is robust and calcium‐independent

FEBS Letters, EarlyView.
Calpains dimerize via penta‐EF‐hand (PEF) domains. Using single‐molecule force spectroscopy, we measured the strength and kinetics of PEF–PEF homodimer binding. The interaction is robust, shows a transient conformational step before dissociation, and remains largely insensitive to Ca2+.
Nesha May O. Andoy, Trina Dykstra‐MacPherson, Mathias A. Bell, Peter L. Davies, Ruby May A. Sullan   +4 more
wiley   +1 more source

HJT-sRNA-m7 bencaosome modulates fibrosis progression in a silicosis mouse model [PDF]

Jichu yixue yu linchuang
Objective To explore the role and mechanism of HJT-sRNA-m7 (M7) bencaosome in a silicosis mouse model. Methods C57BL/6J mice were randomly divided into four groups: blank, control, negative control (NC) oligonucleotide, and M7 treatment (HJT-sRNA-m7 ...
CHANG Jiahui, REN Pengju, ZHOU Yunyi, JIANG Chengyu, ZHANG Yanli
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

LncRNA Snhg3 aggravates hepatic steatosis via PPARγ signaling

eLife
LncRNAs are involved in modulating the individual risk and the severity of progression in metabolic dysfunction-associated fatty liver disease (MASLD), but their precise roles remain largely unknown.
Xianghong Xie, Mingyue Gao, Wei Zhao, Chunmei Li, Weihong Zhang, Jiahui Yang, Yinliang Zhang, Enhui Chen, Yanfang Guo, Zeyu Guo, Minglong Zhang, Ebenezeri Erasto Ngowi, Heping Wang, Xiaoman Wang, Yinghan Zhu, Yiting Wang, Xiaolu Li, Hong Yao, Li Yan, Fude Fang, Meixia Li, Aijun Qiao, Xiaojun Liu   +22 more
doaj   +1 more source

Study of dynamic transcriptome during mitosis of HAP1 cells [PDF]

Jichu yixue yu linchuang
Objective To comprehensively understand the map of transcripts during mitosis and their regulatory mechanisms of HAP1 cells by conducting transcriptome sequencing analysis after being released by mitotic synchronization arrest.
WU Xueli, YANG Yurong, BAO Yongli, WU Rucheng, WANG Lisha, CHEN Yang
doaj   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source

Integrating network pharmacology, molecular docking and experimental verification to explore the protective effect of Buyang Huanwu Decoction on cerebral ischemic stroke

Results in Chemistry
Cerebral ischemic stroke (CIS), the most prevalent form of stroke worldwide, exerts substantial impacts on both public health and socioeconomic conditions, particularly among elderly populations.
Yixuan Guo, Wenqian Liu, Bingsen Qi, Ziyi Liu, Jiaxin Wang, Le Yang, Jingcun Sun, Jinlong Qi   +7 more
doaj   +1 more source

Heat-induced denaturation of cataract-related human γD-crystallin [PDF]

Jichu yixue yu linchuang
Objective To reveal the thermally induced denaturation of wild-type human γD-crystallin(HGD) and congenital cataract-related mutant (HGD P23T), and compare the differences in the structural changes between wild-type and mutants during a heating process.
ZHOU Xin, LI Zhenyan, LI Shuyuan, ZHANG Wenbo, WANG Chenxuan
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Valosin‐containing protein counteracts ATP‐driven dissolution of FUS condensates through its ATPase activity in vitro

FEBS Letters, EarlyView.
Biomolecular condensates formed by fused in sarcoma (FUS) are dissolved by high ATP concentrations yet persist in cells. Using a reconstituted system, we demonstrate that valosin‐containing protein (VCP), an AAA+ ATPase, counteracts ATP‐driven dissolution of FUS condensates through its D2 ATPase activity.
Hitomi Kimura, Shin‐ichi Tate, Kyota Yasuda   +2 more
wiley   +1 more source