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Proteomics of Medicago sativa cell walls
Phytochemistry, 2004A method for the sequential extraction and profiling by two-dimensional gel electrophoresis (2-DE) of Medicago sativa (alfalfa) stem cell wall proteins is described. Protein extraction included freezing, grinding in a sodium acetate buffer, separation by filtration of cell walls from cytosolic contents, and extensive washing.
Bonnie S, Watson +3 more
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Paternal inheritance of plastids in Medicago sativa
Theoretical and Applied Genetics, 1989Plastids are plant cellular organelles that are generally inherited from the maternal parent in the angiosperms. Many species exhibit biparental inheritance of plastids, but usually with a predominantly maternal influence. In contrast to this, we report strong paternal inheritance of plastids in reciprocal crosses of alfalfa, Medicago sativa, by ...
C M, Schumann, J F, Hancock
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Bioaccumulation and degradation of pentachloronitrobenzene in Medicago sativa
Journal of Environmental Management, 2013Pentachloronitrobenzene (PCNB) is a fungicide belonging to the organochlorine family and used extensively in agriculture for crop production. Many studies have implied that PCNB has become an environmental concern due to its widespread contamination in eco-systems.
Ying Ying, Li, Hong, Yang
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Polyploidization and Gene Expression in Medicago sativa
2010Polyploidization is a common event in plant evolution and can influence economically important traits. Modifications of gene expression and/or DNA sequence are known to occur as a consequence of polyploidization. To gain insight into the effects of sexual polyploidization on gene expression in alfalfa, we have used two diploid (2x=16) plants of the ...
CAPOMACCIO, STEFANO +2 more
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Somatic Hybrization of Medicago Sativa and Medicago Falcata
1983The aim of our work was to study somatic hybridization in the genus Medicago. Indeed we would like to increase variability in cultivated lucerne, M. sativa, by introducing characters of wild species. Since interspecific hybridizations are very difficult because of differences in ploidy levels we think that somatic hybridization is a way to overpass ...
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Canavanine in alfalfa (Medicago sativa)
Experientia, 1985Canavanine was extracted and characterized from various cultivars of alfalfa seed. Values obtained range from 8 g/kg for the Buffalo variety to 14–20 g/kg for the highly weevil resistant variety, Weevl-Chek.
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Methods in molecular biology (Clifton, N.J.), 2006
A protocol for rapid, highly efficient transformation of alfalfa is described. Leaf explants from growth chamber-grown plants of a highly regenerable genotype are surface-sterilized, the margins are removed, and explants are inoculated with Agrobacterium tumefaciens strain LBA4404 carrying the T-DNA vector of interest.
Deborah A, Samac +1 more
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A protocol for rapid, highly efficient transformation of alfalfa is described. Leaf explants from growth chamber-grown plants of a highly regenerable genotype are surface-sterilized, the margins are removed, and explants are inoculated with Agrobacterium tumefaciens strain LBA4404 carrying the T-DNA vector of interest.
Deborah A, Samac +1 more
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