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Proteomics of Medicago sativa cell walls
Phytochemistry, 2004A method for the sequential extraction and profiling by two-dimensional gel electrophoresis (2-DE) of Medicago sativa (alfalfa) stem cell wall proteins is described. Protein extraction included freezing, grinding in a sodium acetate buffer, separation by filtration of cell walls from cytosolic contents, and extensive washing.
Bonnie S, Watson +3 more
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Medicago sativa subsp. sativa Linnaeus 1753
2007Published as part of Jarvis, Charlie, 2007, Chapter 7: Linnaean Plant Names and their Types (part M), pp. 651-689 in Order out of Chaos. Linnaean Plant Types and their Types, London :Linnaean Society of London in association with the Natural History Museum on page 661, DOI: 10.5281/zenodo ...
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Methods in molecular biology (Clifton, N.J.), 2006
A protocol for rapid, highly efficient transformation of alfalfa is described. Leaf explants from growth chamber-grown plants of a highly regenerable genotype are surface-sterilized, the margins are removed, and explants are inoculated with Agrobacterium tumefaciens strain LBA4404 carrying the T-DNA vector of interest.
Deborah A, Samac +1 more
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A protocol for rapid, highly efficient transformation of alfalfa is described. Leaf explants from growth chamber-grown plants of a highly regenerable genotype are surface-sterilized, the margins are removed, and explants are inoculated with Agrobacterium tumefaciens strain LBA4404 carrying the T-DNA vector of interest.
Deborah A, Samac +1 more
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Genetic Engineering of Alfalfa (Medicago sativa L.)
Protein & Peptide Letters, 2016Alfalfa is excellent perennial legume forage for its extensive ecological adaptability, high nutrition value, palatability and biological nitrogen fixation. It plays a very important role in the agriculture, animal husbandry and ecological construction. It is cultivated in all continents.
Dan, Wang +5 more
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Canavanine in alfalfa (Medicago sativa)
Experientia, 1985Canavanine was extracted and characterized from various cultivars of alfalfa seed. Values obtained range from 8 g/kg for the Buffalo variety to 14–20 g/kg for the highly weevil resistant variety, Weevl-Chek.
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Haploids from Cultivated Alfalfa, Medicago sativa L.
Nature, 1969HAPLOIDS are valuable for new approaches to genetic, cytogenetic and breeding research in plants such as cultivated alfalfa, Medicago sativa L., which behave as auto-tetraploids. Their use is limited in this species, however, by the lack of a technique for their isolation; only three haploids have been reported1–3. Recently, we isolated eight haploids (
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