Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Perception of medical students and interns toward orthopedics as a future career in Saudi Arabia [PDF]
Hassan A Al-Ghanim +5 more
openalex +1 more source
Amino acids sequence of two different proteins with the same sequence (chameleon sequence—black boxes) represent in 3D structure of the proteins different secondary structures: HHHH—helical and BBB—Beta‐structural. The chains folded in water environment adopt different III‐order structures in which the chameleon fragments appear to adopt similar status
Irena Roterman +4 more
wiley +1 more source
Stress and career aspirations: a longitudinal study with medical students
BackgroundThe psychological well-being of medical students has often been studied in relation to various factors and typically exhibits poorer scores than comparable student groups.
Clara Picker-Roesch, Jessica Lang
doaj +1 more source
Perceived factors of choosing anesthesiology as a career among medical students in Umm Al-Qura University: A cross-sectional study [PDF]
Salah Bakry +9 more
openalex +1 more source
The Net Present Value and Other Economic Implications of a Medical Career.
U. Reinhardt
semanticscholar +1 more source
Screening and epitope characterization of Nidogen‐2‐specific nanobodies
Camel immunization and phage display were employed to generate high‐affinity VHH nanobodies against Nidogen‐2. After library construction, biopanning, ELISA screening, sequencing, and recombinant expression, selected nanobodies were purified and characterized, leading to the preliminary exploration of a nanobody‐based sandwich ELISA for specific ...
Jianchuan Wen +9 more
wiley +1 more source
Cutaneous Melanoma Drives Metabolic Changes in the Aged Bone Marrow Immune Microenvironment
Melanoma, the deadliest form of skin cancer, increasingly affects older adults. Our study reveals that melanoma induces changes in iron and lipid levels in the bone marrow, impacting immune cell populations and increasing susceptibility to ferroptosis.
Alexis E. Carey +12 more
wiley +1 more source
The study investigates the influence of career calling on the learning engagement of medical students in higher vocational colleges, focusing on the chain mediation roles of career decision self-efficacy (CDSE) and career adaptability.
Jingyuan Chen +3 more
doaj +1 more source
A Realist Evaluation of Theory about Triggers for Doctors Choosing a Generalist or Specialist Medical Career. [PDF]
O'Sullivan B +3 more
europepmc +1 more source

