Results 91 to 100 of about 1,257,317 (293)

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

open access: yesFEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen   +5 more
wiley   +1 more source

Magnetic properties and magnetocaloric effects of rare-earth based high-entropy amorphous microwires

open access: yesJournal of Materials Research and Technology
The development of efficient and environmentally friendly solid-state refrigeration materials is a key issue in magnetic refrigeration technology. Rare-earth based high-entropy alloys with good magnetocaloric effect have been recently recognized as good ...
Shijie Wei   +4 more
doaj   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

open access: yesFEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh   +2 more
wiley   +1 more source

A General Model for the Permeability of Magma Mush

open access: yesGeochemistry, Geophysics, Geosystems
Percolation through magma mush is a key transport mechanism for melts in the crust and is influenced by the permeability of the crystal framework. Existing models for mush permeability do not account for the range of microstructures that can evolve as ...
Eloïse Bretagne   +5 more
doaj   +1 more source

Influence of High-Temperature Treatment of Melt on the Composition and Structure of Aluminum Alloy

open access: yesArchives of Foundry Engineering, 2017
The aim of the current study was to examine the structure of an alloy treated at various temperatures up to 2,000-2,100 °C. Among research techniques for studying alloy structure there were the electron and optical microstructure, X-ray structure, and ...
Grachev V. A., Turakhodjaev N. D.
doaj   +1 more source

The C‐terminal domain of yeast Arginyltransferase1 is essential for its catalytic activity

open access: yesFEBS Open Bio, EarlyView.
Arginyltransferase 1 (Ate1), a eukaryotic enzyme, catalyses arginylation, transferring arginine from tRNA‐Arg to the amino terminus of the target protein. Overexpression of Ate1 in yeast is lethal and is dependent on arginylation. This study elucidates how mutations in the cofactor‐binding and active site of Ate1 and truncation of its structural ...
Vikas Kumar Yadav   +4 more
wiley   +1 more source

Thermodynamic Modeling of Lithium Enrichment During Partial Melting: The Importance of Partition Coefficients

open access: yesGeochemistry, Geophysics, Geosystems
Predicting the partial melting conditions that generate Li‐rich peraluminous melts is of major economic and societal importance. However, existing models disagree on the optimal conditions (e.g., pressure, temperature, protolith) and, consequently, on ...
Nicolas Riel   +6 more
doaj   +1 more source

MARK4 enhances stress granule formation under oxidative stress and increases tau accumulation

open access: yesFEBS Open Bio, EarlyView.
MARK4 (red dots) localizes to stress granules (orange dots) and promotes their formation under oxidative stress by modulating TIA1 (blue dots). MARK4 and TIA1 synergistically increase tau (purple) accumulation, and the reduction of the TIA1 ortholog suppresses neurodegeneration in a fly model.
Sho Nakajima   +8 more
wiley   +1 more source

Parametric Studies of Melt Electrospinning Poly ?(caprolactone) Fibers for Tissue Engineering Applications [PDF]

open access: yes, 2013
The process of electrospinning has received remarkable amount of attention as this technique can be used to fabricate suitable scaffolds for cells.
Ko, Junghyuk   +4 more
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