Results 51 to 60 of about 12,198,711 (338)

Reconstructing enzyme evolution by protein engineering

open access: yesFEBS Letters, EarlyView.
Natural enzyme evolution can be retraced by protein engineering methods such as directed evolution, rational design, and ancestral sequence reconstruction. These approaches reveal how enzymes emerged from ligand‐binding scaffolds, developed varying substrate preferences, formed oligomeric complexes, adapted to environmental changes, and evolved novel ...
Lukas Drexler   +2 more
wiley   +1 more source

Simulation-based optimization of PCM melting temperature to improve the energy performance in buildings

open access: yes, 2017
Globally, a considerable amount of energy is consumed by the building sector. The building envelope can highly influence the energy consumption in buildings.
M. Saffari   +3 more
semanticscholar   +1 more source

The Shewanella oneidensis Fic enzyme SoFic targets the switch‐I region of EF‐Tu for AMPylation

open access: yesFEBS Letters, EarlyView.
Fic enzymes mediate diverse post‐translational modifications across all domains of life, including AMPylation. Prokaryotic EF‐Tu can be AMPylated and deAMPylated by the conserved Fic enzyme SoFic. Structural and biochemical approaches were used to characterize the effect of AMPylation on EF‐Tu, SoFic's enzymatic activities, and the enzyme‐target ...
Svenja Runge   +6 more
wiley   +1 more source

A melting temperature database and a neural network model for melting temperature prediction

open access: yes, 2021
I build a melting temperature database that contains approximately 10,000 materials. Based on the database, I build a machine learning model that predicts melting temperature in seconds. The model features graph neural network and residual neural network architecture.
openaire   +2 more sources

Calculating melting temperature [PDF]

open access: yesNature, 1986
Some journals still allow their contributors leisure, which makes for a readable account of a calculation of the Earth's inner core.
openaire   +1 more source

MELTING, a flexible platform to predict the melting temperatures of nucleic acids [PDF]

open access: yesBMC Bioinformatics, 2012
Computing accurate nucleic acid melting temperatures has become a crucial step for the efficiency and the optimisation of numerous molecular biology techniques such as in situ hybridization, PCR, antigene targeting, and microarrays. MELTING is a free open source software which computes the enthalpy, entropy and melting temperature of nucleic acids ...
Marine Dumousseau   +3 more
openaire   +4 more sources

Influence of Ga Content on the Microstructure and Mechanical Properties of Cadmium-Free Filler Metal

open access: yesMetals, 2022
The influence of Ga content on the melting temperature, wettability, microstructure, and mechanical properties of low-silver 12AgCuZnSn-2In-0.15Pr cadmium-free filler metal was investigated systematically by means of differential thermal analysis, X-ray ...
Jie Wu   +4 more
doaj   +1 more source

Global glacier change in the 21st century: Every increase in temperature matters

open access: yesScience, 2023
Glacier mass loss affects sea level rise, water resources, and natural hazards. We present global glacier projections, excluding the ice sheets, for shared socioeconomic pathways calibrated with data for each glacier.
D. Rounce   +12 more
semanticscholar   +1 more source

Stability of (Mg,Fe)SiO3 perovskite and the structure of the lowermost mantle [PDF]

open access: yes, 1992
Thermodynamic analysis shows that (Mg,Fe)SiO3 perovskite is stable throughout the likely pressure, temperature and compositional regime of the Earth's mantle.
Bukowinski, MST, Stixrude, L
core  

L‐aspartate oxidase provides new insights into fumarate reduction in anaerobic darkness in Synechocystis sp. PCC6803

open access: yesFEBS Letters, EarlyView.
Synechocystis strains deficient in succinate dehydrogenase (SDH) secrete more succinate than the WT under dark anaerobic conditions, supporting that SDH then primarily acts as SDH, not as a fumarate reductase. L‐aspartate oxidase (Laspo) from Synechocystis is functional under anaerobic conditions, reducing fumarate to succinate.
Kateryna Kukil   +3 more
wiley   +1 more source

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