Results 41 to 50 of about 126,108 (313)

Machine learning for identifying liver and pancreas cancers through comprehensive serum glycopeptide spectra analysis: a case‐control study

Molecular Oncology, EarlyView.
This study presents a novel AI‐based diagnostic approach—comprehensive serum glycopeptide spectra analysis (CSGSA)—that integrates tumor markers and enriched glycopeptides from serum. Using a neural network model, this method accurately distinguishes liver and pancreatic cancers from healthy individuals.
Motoyuki Kohjima, Yuko Takami, Ken Kawabe, Kazuhiro Tanabe, Chihiro Hayashi, Mikio Mikami, Tetsuya Kusumoto   +6 more
wiley   +1 more source

Identification of N-Glycoproteins of Knee Cartilage from Adult Osteoarthritis and Kashin-Beck Disease Based on Quantitative Glycoproteomics, Compared with Normal Control Cartilage

Cells, 2022
Glycoproteins are involved in the development of many diseases, while the type and content of N-glycoproteins in the cartilage of osteoarthritis (OA) and Kashin–Beck disease (KBD) are still unclear.
Jing Han, Huan Deng, Yizhen Lyu, Xiang Xiao, Yan Zhao, Jiaxin Liu, Ziwei Guo, Xuan Liu, Lichun Qiao, Hang Gao, Mikko Juhani Lammi   +10 more
doaj   +1 more source

Unveiling unique protein and phosphorylation signatures in lung adenocarcinomas with and without ALK, EGFR, and KRAS genetic alterations

Molecular Oncology, EarlyView.
Proteomic and phosphoproteomic analyses were performed on lung adenocarcinoma (LUAD) tumors with EGFR, KRAS, or EML4–ALK alterations and wild‐type cases. Distinct protein expression and phosphorylation patterns were identified, especially in EGFR‐mutated tumors. Key altered pathways included vesicle transport and RNA splicing.
Fanni Bugyi, Mirjam Balbisi, Simon Sugár, Lóránd Váncza, Eszter Regős, Ilona Kovalszky, Ibolya Laczó, Tünde Harkó, Gábor Kecskeméti, Zoltán Szabó, Judit Moldvay, László Drahos, Lilla Turiák   +12 more
wiley   +1 more source

Transient Transfection-based Fusion Assay for Viral Proteins

Bio-Protocol, 2017
Membrane fusion is vital for entry of enveloped viruses into host cells as well as for direct viral cell-to-cell spread. To understand the fusion mechanism in more detail, we use an infection free system whereby fusion can be induced by a minimal set of ...
Melina Vallbracht, Christina Schröter, Barbara Klupp, Thomas Mettenleiter   +3 more
doaj   +1 more source

Challenging the Existing Model of the Hexameric HIV-1 Gag Lattice and MA Shell Superstructure: Implications for Viral Entry

Viruses, 2021
Despite type 1 human immunodeficiency virus (HIV-1) being discovered in the early 1980s, significant knowledge gaps remain in our understanding of the superstructure of the HIV-1 matrix (MA) shell.
Joy Ramielle L. Santos, Weijie Sun, Tarana A. Mangukia, Eduardo Reyes-Serratos, Marcelo Marcet-Palacios   +4 more
doaj   +1 more source

Protein O‐glycosylation in the Bacteroidota phylum

FEBS Open Bio, EarlyView.
Species of the Bacteroidota phylum exhibit a unique O‐glycosylation system. It modifies noncytoplasmic proteins on a specific amino acid motif with a shared glycan core but a species‐specific outer glycan. A locus of multiple glycosyltransferases responsible for the synthesis of the outer glycan has been identified.
Lonneke Hoffmanns, Dennis Svedberg, André Mateus   +2 more
wiley   +1 more source

Retinal glycoprotein enrichment by concanavalin a enabled identification of novel membrane autoantigen synaptotagmin-1 in equine recurrent uveitis. [PDF]

PLoS ONE, 2012
Complete knowledge of autoantigen spectra is crucial for understanding pathomechanisms of autoimmune diseases like equine recurrent uveitis (ERU), a spontaneous model for human autoimmune uveitis. While several ERU autoantigens were identified previously,
Margarete E Swadzba, Stefanie M Hauck, Hassan Y Naim, Barbara Amann, Cornelia A Deeg   +4 more
doaj   +1 more source

Matrigel inhibits elongation and drives endoderm differentiation in aggregates of mouse embryonic stem cells

FEBS Open Bio, EarlyView.
Stem cell‐based embryo models (SCBEMs) are valuable to study early developmental milestones. Matrigel, a basement membrane matrix, is a critical substrate used in various SCBEM protocols, but its role in driving stem cell lineage commitment is not clearly defined.
Atoosa Amel, Rachel Brown, Alexa Rabeling, Mubeen Goolam   +3 more
wiley   +1 more source

N-glycoproteome analysis of the secretome of human metastatic hepatocellular carcinoma cell lines combining hydrazide chemistry, HILIC enrichment and mass spectrometry. [PDF]

PLoS ONE, 2013
Cancer cell metastasis is a major cause of cancer death. Unfortunately, the underlying molecular mechanisms remain unknown, which results in the lack of efficient diagnosis, therapy and prevention approaches. Nevertheless, the dysregulation of the cancer
Xianyu Li, Jing Jiang, Xinyuan Zhao, Jifeng Wang, Huanhuan Han, Yan Zhao, Bo Peng, Rugang Zhong, Wantao Ying, Xiaohong Qian   +9 more
doaj   +1 more source

Linkage of a membrane skeleton to integral membrane glycoproteins in human platelets. Identification of one of the glycoproteins as glycoprotein Ib. [PDF]

Journal of Clinical Investigation, 1985
Experiments were performed to determine whether platelets contain a membrane skeleton. Platelets were labeled by a sodium periodate/sodium [3H]borohydride method and lysed with Triton X-100. Much of the filamentous actin could be sedimented at low g forces (15,600 g, 4 min), but some of the actin filaments required high-speed centrifugation for their ...
openaire   +3 more sources