Results 261 to 270 of about 704,164 (306)

Mitochondrial Membrane Potential Assay

2022
Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in various types of cells, such as HepG2 ...
Srilatha, Sakamuru, Jinghua, Zhao, Matias S, Attene-Ramos, Menghang, Xia   +3 more
openaire   +2 more sources

Longevity and mitochondrial membrane potential

Biochemistry (Moscow), 2012
In Saccharomyces cerevisiae yeast cells a decrease in the mitochondrial membrane potential caused by protonophores or by a loss of mitochondrial DNA leads to an increase in longevity (replicative life span). The loss of mitochondrial DNA also activates retrograde signaling that results in certain changes in transcription. Recently, Miceli and coauthors
D A, Knorre, F F, Severin
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Mitochondrial membrane potentials in ischemic hearts

Archives of Biochemistry and Biophysics, 2003
Excised rat hearts were perfused isovolumically and then made globally ischemic for times varying from 0 to 70 min followed by 50 min of reperfusion. In situ mitochondrial electrical potential gradients (Deltapsi(m)) were measured during reperfusion using the lipophilic cation, 3H-tetraphenylphosphonium.
Deborah A, Berkich, Guy, Salama, Kathryn F, LaNoue   +2 more
openaire   +2 more sources

Probing mitochondrial membrane potential

C&EN Global Enterprise, 2020
Mitochondrial membrane potential, an indicator of mitochondrial function, is perturbed in many diseases , including cancer and neurodegenerative diseases, but difficult to measure noninvasively.
openaire   +1 more source

Quantification of Myocardial Mitochondrial Membrane Potential Using PET

Current Cardiology Reports, 2021
To present a method enabling in vivo quantification of tissue membrane potential (ΔΨT), a proxy of mitochondrial membrane potential (ΔΨm), to review the origin and role of ΔΨm, and to highlight potential applications of myocardial ΔΨT imaging.Radiolabelled lipophilic cations have been used for decades to measure ΔΨm in vitro.
Matthieu, Pelletier-Galarneau, Felicitas J, Detmer, Yoann, Petibon, Marc, Normandin, Chao, Ma, Nathaniel M, Alpert, Georges, El Fakhri   +6 more
openaire   +2 more sources

Mitochondrial membrane potential (MMP) regulates sperm motility

In Vitro Cellular & Developmental Biology - Animal, 2016
Sperm motility is the major decisive factor in determining male fertility. The objective of the present study was to analyse the effect of mitochondrial membrane potential (MMP) on the temporal regulation of sperm motility. Observations were recorded in various rodent species and among differentially motile sperm fractions including swim up and ...
Saurabh Kumar, Agnihotri, Ankit Kumar, Agrawal, Bilal Ahmad, Hakim, A L, Vishwakarma, T, Narender, Rekha, Sachan, Monika, Sachdev   +6 more
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Protein unfolding by the mitochondrial membrane potential

Nature Structural Biology, 2002
Mitochondria can unfold importing precursor proteins by unraveling them from their N-termini. However, how this unraveling is induced is not known. Two candidates for the unfolding activity are the electrical potential across the inner mitochondrial membrane and mitochondrial Hsp70 in the matrix.
Shihai, Huang, Kevin S, Ratliff, Andreas, Matouschek   +2 more
openaire   +2 more sources

Membrane Potential of Mitochondrial Measured with Microelectrodes

Science, 1977
The membrane potentials of giant mitochondria from cuprizone-fed mice were found to be independent of metabolic state. Experiments are described in which the presence of the microelectrodes in the inner mitochondrial space, and the metabolic viability of the impaled mitochonidra, are validated.
B L, Maloff, S P, Scordilis, H, Tedeschi
openaire   +2 more sources