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Preservation of Mitochondrial Membrane Potential During Anoxia

1996
Mitochondria (Mt) in living cells can be intensely and selectively stained against a dark cytoplasmic background by several fluorescent cationic dyes. For example, the incorporation of Rhodamine 123 (RH123) into Mt depends on the membrane potential, accompanied by red shifts of absorption spectra and quenching of fluorescence intensity (Emaus et al ...
Y, Nomura, T, Miyao, M, Tamura
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The plasma membrane and mitochondrial membrane potentials of Plasmodium yoelii

Comparative Biochemistry and Physiology Part B: Comparative Biochemistry, 1988
1. The plasma membrane potential and the mitochondrial membrane potential of P. yoellii was examined by fluorescence microscopy using rhodamine 123 and by transmembrane distribution of tetraphenylphosphonium. 2. The mitochondrion of P. yoelii, free of gametocyte stage, maintained a high negative inside membrane potential. 3.
A, Izumo, K, Tanabe, M, Kato
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Mitochondrial membrane potential and ischemic neuronal death

Neuroscience Research, 2006
Mitochondria are intracellular organelles in which high energy phosphate is produced. Ischemia causes depletion of the materials necessary to produce this phosphate and strongly affects the electron transport chain. Apoptosis commences during and after ischemia.
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Relation Between Mitochondrial Membrane Potential and ROS Formation

2011
Mitochondria are considered the main source of reactive oxygen species (ROS) in the cell. For this reason they have been recognized as a source of various pathological conditions as well as aging. Chronic increase in the rate of ROS production is responsible for the accumulation of ROS-associated damages in DNA, proteins, and lipids and may result in ...
Suski JM   +5 more
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Mitochondrial membrane potential and ageing in Podospora anserina

Mechanisms of Ageing and Development, 2001
Some filamentous fungi exhibit a limited vegetative growth with modifications in the mitochondria, suggesting the involvement of mitochondria in the process of ageing. Nevertheless, the relationship between the ability to grow or the fate of these cells relative to their mitochondrial membrane potential (Psi(mt)) level has not been investigated.
F, Koll   +3 more
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Visualization of mitochondrial membrane potential in mammalian cells

2020
Assessment of the mitochondrial membrane potential (Δψ) in living cells, although not trivial, can be used to estimate mitochondrial bioenergetic state. For this purpose, fluorescent lipophilic cations are broadly applied. These cations enter cells and accumulate primarily in the mitochondrial matrix in a Δψ-dependent manner.
Esteras, N.   +4 more
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UCP2 muscle gene transfer modifies mitochondrial membrane potential

International Journal of Obesity, 2001
The aim of this work was to evaluate the effect of uncoupling protein 2 (UCP2) muscle gene transfer on mitochondrial activity.Five week-old male Wistar rats received an intramuscular injection of plasmid pXU1 containing UCP2 cDNA in the right tibialis anterior muscles. Left tibialis anterior muscles were injected with vehicle as control. Ten days after
A, Marti   +4 more
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Mitochondrial membrane potential after low-power laser irradiation

Lasers in Medical Science, 2004
We used the lipophilic cationic fluorescent dye 5,5',6,6'-tetrachloro-1,1',3,3'-tetraethyl-benzimidazol-carbocyanine iodide (JC-1) to determine mitochondrial membrane potential (mdeltapsi) in Hep-2 cells after irradiation with low-power laser (lambda=635 nm).
R, Bortoletto   +5 more
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Mitochondrial Membrane Potential and Dynamics

2011
Mitochondria are essential for neuronal homeostasis and their dysfunction causes neurodegeneration. Mitochondrial bioenergetics and dynamics ensure ATP supply and Ca2+ buffering throughout neuronal processes, regulating and being reciprocally modulated by synaptic activity.
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Preserved motor axonal membrane potential in mitochondrial disease

Journal of Neurology, Neurosurgery & Psychiatry, 2010
Mitochondrial disorders are characterised by protean neurological manifestations including peripheral neuropathy. The neuropathy is typically an axonal process, with a proposed mechanism being degradation of the membrane potential of the axon due to dysfunction of energy-dependent Na(+)/K(+) ATPase pump.
K, Ng, S, Winter, C, Sue, D, Burke
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