Results 281 to 290 of about 14,693,728 (394)

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

open access: yesFEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

TbTim20 facilitates protein import at a low membrane potential in trypanosomes lacking the mitochondrial genome

open access: yes
von Känel C   +8 more
europepmc   +1 more source

A red-emitting carborhodamine for monitoring and measuring membrane potential. [PDF]

open access: yesProc Natl Acad Sci U S A
Gest AMM   +5 more
europepmc   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

open access: yesFEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa   +2 more
wiley   +1 more source

Matrix: a complex amalgam of structures and functions in tumor microenvironment

open access: yesFEBS Open Bio, EarlyView.
The matrix is a dynamic, intricate three‐dimensional mesh of biomolecules with both structural and functional properties. This review deals with the complexity of this ‘molecular amalgam’ in the tumor microenvironment and highlights its importance in the maintenance and evolution of tumors by describing certain matrix biomolecules, such as ...
Spyros S. Skandalis   +3 more
wiley   +1 more source

Mitochondrial Membrane Potential of CD8<sup>+</sup> T Cells Predicts Bacterial Infection and Rapid Development of Acute-on-chronic Liver Failure in Cirrhotic Patients. [PDF]

open access: yesJ Clin Transl Hepatol
Wang X   +13 more
europepmc   +1 more source

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