Results 241 to 250 of about 1,708,376 (302)

The Effects of CuSO₄ on Membrane Potential and Glutamatergic Synaptic Transmission. [PDF]

Biology (Basel)
Peters S, Neglia K, Bierbower SM, Cooper RL.   +3 more
europepmc   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

μ-Opioid receptor signalling enhances Kir3 currents in glutamatergic preBötzinger complex neurons in mice. [PDF]

J Physiol
Kalajian EJ, Stettler MK, Smith GDC, Del Negro CA.   +3 more
europepmc   +1 more source

Protocol for quantifying miRNA trafficking across the endosomal membrane

FEBS Open Bio, EarlyView.
An in vitro protocol measures miRNA uptake into endosomes isolated from mammalian cell extracts, which are free of subcellular contaminants. Performed at 37 °C in the presence of ATP, it ensures the import of single‐stranded miRNA into the endosomal lumen.
Syamantak Ghosh, Kamalika Mukherjee, Suvendra N. Bhattacharyya   +2 more
wiley   +1 more source

Insights into the Ir-O redox dynamics of an operating PEM water electrolyzer using in operando Raman spectroscopy. [PDF]

Commun Chem
Speer S, Jovanovic S, Oeß C, Karl A, Jodat E, Eichel RA.   +5 more
europepmc   +1 more source

Structural and biochemical insights into the thermostable esterase Ta0887 from Thermoplasma acidophilum

FEBS Open Bio, EarlyView.
In this study, a novel esterase from the thermoacidophilic archaeon Thermoplasma acidophilum was biochemically and structurally characterized. Our results demonstrate that Ta0887 is a highly thermostable esterase that preferentially hydrolyzes p‐nitrophenyl hexanoate and possesses an α‐helical cap domain that likely contributes to its substrate ...
Alejandro Delgado‐Rey, Miriam Livier Llamas‐García, Gabriela M. Montero‐Morán, Leticia Santos, Samuel Lara‐González   +4 more
wiley   +1 more source

Human iPSC-Derived Spinal Cord Organoids - The First Electrophysiological Assessment of Neuronal Maturation. [PDF]

ACS Chem Neurosci
Ievglevskyi O, Zosen D, Osete JR, Glover JC, Elisabeth Paulsen R, Djurovic S, Kondratskaya E.   +6 more
europepmc   +1 more source

Threonine 348 regulates the subcellular localization of PTEN

FEBS Open Bio, EarlyView.
Thr348 in the C2 domain is a key contributor to PTEN subcellular localization. The PTEN350 fragment and PTENA4 accumulated in the nucleus, whereas PTENK13R,A4 predominantly localized to the plasma membrane. In contrast, substitution of Thr348 with Asp (T348D) disrupted these characteristic localization patterns, resulting in predominant cytoplasmic ...
Takashi Kato, Suzu Tanaka, Miyu Ohashi
wiley   +1 more source

Analysis of dendritic input currents during place field dynamics. [PDF]

Elife
Fogel B, Ujfalussy BB.
europepmc   +1 more source

Melatonin suppresses abnormal hyperexcitability of hippocampal CA1 neurons in early-stage oxygen-glucose deprivation. [PDF]

Front Pharmacol
Cui Y, Wang Q, Liu C, Zhou H.
europepmc   +1 more source