Results 121 to 130 of about 10,936,171 (356)

CryoEM screening with a blotting instrument: quantifying parameters affecting ice thickness using semi-automated image analysis

Methods in Microscopy
Cryo-electron microscopy (cryoEM) grid preparation is one of the bottlenecks in using cryoEM for macromolecular structure determination. Despite significant advancements in the development of blot-free instruments, their high cost limits their widespread
Valimehr Sepideh, Cho Ellie, Brown Hamish G., McMillan Paul J., Hanssen Eric   +4 more
doaj   +1 more source

A New method for the capture of surface proteins in Plasmodium falciparum parasitized erythrocyte [PDF]

, 2012
Introduction: We propose a new method for the selective labeling, isolation and electrophoretic analysis of the Plasmodium falciparum protein exposed on the erythrocyte cell surface.
Ferru, Emanuela, Pantaleo, Antonella, Turrini, Francesco Michelangelo   +2 more
core  

Protein phosphorylation in yeast mitochondria [PDF]

, 1987
We describe the identification and submitochondrial localization of four protein kinases and of their target proteins in derepressed yeast mitochondria. The activity of one of the kinases depends on the presence of cyclic AMP (cAMP).
Ashwell, Bandlow, Bandlow, Barrera, Bradford, Chandrasekaran, Chock, Daum, De Vendittis, Dimino, Douglas, Eraso, Fang, Heil, Henriksson, Hixson, Kim, Kitagawa, Krebs, Lastick, Linn, Mahler, Mahler, Matsumoto, Matsumoto, Matsumoto, Pall, Racker, Rigobello, Rittenhouse, Rödel, Steinberg, Steinberg, Suissa, Towbin, Van der Plaat, Vardanis, Wang   +37 more
core   +2 more sources

Tau acetylation at K331 has limited impact on tau pathology in vivo

FEBS Letters, EarlyView.
We mapped tau post‐translational modifications in humanized MAPT knock‐in mice and in amyloid‐bearing double knock‐in mice. Acetylation within the repeat domain, particularly around K331, showed modest increases under amyloid pathology. To test functional relevance, we generated MAPTK331Q knock‐in mice.
Shoko Hashimoto, Yukio Matsuba, Mika Takahashi, Takaomi C. Saido   +3 more
wiley   +1 more source

Structure and function of bacterial dynamin-like proteins [PDF]

, 2012
Membrane dynamics are essential for numerous cellular processes in eukaryotic and prokaryotic cells. In eukaryotic cells, membrane fusion and fission are often catalyzed by large GTPases of the dynamin protein family. These proteins couple GTP hydrolysis
Bramkamp, Marc
core   +1 more source

Hyperosmotic stress induces PARP1‐mediated HPF1‐dependent mono(ADP‐ribosyl)ation

FEBS Letters, EarlyView.
Sorbitol‐induced hyperosmotic stress rapidly induces reversible mono(ADP‐ribosyl)ation (MARylation) on PARP1 without the signs of genotoxic signaling. We show that PARP1 autoMARylation is HPF1 dependent and forms hydroxylamine‐resistant O‐glycosidic linkages.
Anna Georgina Kopasz, Mihály Mérey, Rebeka Vásárhelyi, Ramóna Pék, Victor Imburchia, László Henn, Adrián Kószó, Nicholas D. Lakin, Ivan Ahel, Sébastien Huet, Ágnes Czibula, Gyula Timinszky   +11 more
wiley   +1 more source

Tumor cells ectopically expressing the membrane-bound form of IL-7 develop an antitumor immune response efficiently in a colon carcinoma model

Molecules and Cells
: Various approaches employing cytokines and cytokine gene–modified tumor cells have been explored to induce antitumor responses, yet their widespread application has been limited due to efficacy concerns and adverse effects. In this study, interleukin-7
Hee-Su Shin, Hyejin Kim, Soon-Gyu Kwon, Hayyoung Lee, Jie-Oh Lee, Young Sang Kim   +5 more
doaj   +1 more source

Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells [PDF]

, 2016
The asymmetric localization of proteins in the plasma membrane domains of eukaryotic cells is a fundamental manifestation of cell polarity that is central to multicellular organization and developmental patterning.
Friml, Jiri, Langowski, Lukasz, Li, Hongjiang, Naramoto, Satoshi, Tanaka, Hirokazu, Vanneste, Steffen, Wabnik, Krzysztof   +6 more
core   +4 more sources

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Nano-org, a functional resource for single-molecule localisation microscopy data

Nature Communications
The nanoscale organisation of proteins plays a key role in diverse cellular processes, including signalling, adhesion, and structural integrity. Single-molecule localisation microscopy (SMLM) is a super-resolution imaging technique that captures the ...
S. Shirgill, D. J. Nieves, J. A. Pike, M. A. Ahmed, H. Abbott, M. H. H. Baragilly, K. Savoye, J. D. Worboys, K. S. Hazime, E. Bruggeman, A. Garcia, D. J. Williamson, P. Rubin-Delanchy, R. Peters, D. M. Davis, R. Henriques, S. F. Lee, D. M. Owen   +17 more
doaj   +1 more source