Results 251 to 260 of about 8,544,371 (366)

Structure and function of bacterial outer membrane proteins: barrels in a nutshell

Molecular Microbiology, 2000
R. Koebnik, K. Locher, P. Van Gelder
semanticscholar   +1 more source

An efficient strategy for producing RNA‐free Nucleocapsid protein of SARS‐CoV‐2 for biochemical and structural investigations

FEBS Open Bio, EarlyView.
Cleavable N‐terminal Thioredoxin fusion enabled soluble expression and purification of otherwise insoluble SARS‐CoV‐2 Nucleocapsid (N) protein. A four‐step purification strategy yielded highly homogeneous, RNA‐free N protein. Binding assays showed high RNA affinity (Kd ~ 28 nm). The study will facilitate high‐resolution structural studies of N protein,
Shweta Singh, Gagan D. Gupta
wiley   +1 more source

Trafficking of K63-polyubiquitin-modified membrane proteins in a macroautophagy-independent pathway is linked to ATG9A. [PDF]

Mol Biol Cell
Scavone F, Lian S, Eskelinen EL, Cohen RE, Yao T.   +4 more
europepmc   +1 more source

Membrane assembly in vitro : Synthesis, glycosylatio, and asymmetric insertion of a transmembrane protein

, 1977
Flora Katz, James E. Rothman, Vishwanath R. Lingappa, Günter Blobel, Harvey F. Lodish   +4 more
openalex   +1 more source

Analysis of the regulation of undecaprenyl diphosphate dephosphorylation in Escherichia coli

FEBS Open Bio, EarlyView.
BacA, PgpB, and YbjG phosphatases are involved in undecaprenyl phosphate (C55P) synthesis in Escherichia coli. We analyzed the lipid contents and the gene expression in the gene‐disruption strains. Undecaprenyl diphosphate (C55PP) level increased in the bacA, ybjG double‐disruption strain, but C55P levels were similar in all strains.
Tomotaka Jitsukawa, Yasushi Shigeri, Shingo Fujisaki   +2 more
wiley   +1 more source

MPicker: visualizing and picking membrane proteins for cryo-electron tomography. [PDF]

Nat Commun
Yan X, Li S, Huang W, Wang H, Zhao T, Huang M, Zhou N, Shen Y, Li X.   +8 more
europepmc   +1 more source

Functional parameters of spermatozoa obtained by a new selection device

FEBS Open Bio, EarlyView.
This study compares density gradient centrifugation and the LensHooke CA0™ device for sperm separation. DGC requires multiple steps, specialized equipment, and technical expertise. In contrast, the CA0 device offers key practical and methodological advantages, resulting in a faster, simpler, and more affordable sperm selection method.
Julio C. Chávez, Paulina Torres, Gabriela Carrasquel‐Martínez, María G. Figueroa‐Méndez, Diana Flores, Lina Villar, Israel Maldonado, Marcela B. Trevino, Claudia Treviño   +8 more
wiley   +1 more source

Advances in utilizing reverse micelles to investigate membrane proteins. [PDF]

Biochem Soc Trans
Walters SH, Birchfield AS, Fuglestad B.
europepmc   +1 more source

Membrane lipids: where they are and how they behave

Nature reviews. Molecular cell biology, 2008
G. Meer, D. Voelker, G. Feigenson
semanticscholar   +1 more source

Matrix: a complex amalgam of structures and functions in tumor microenvironment

FEBS Open Bio, EarlyView.
The matrix is a dynamic, intricate three‐dimensional mesh of biomolecules with both structural and functional properties. This review deals with the complexity of this ‘molecular amalgam’ in the tumor microenvironment and highlights its importance in the maintenance and evolution of tumors by describing certain matrix biomolecules, such as ...
Spyros S. Skandalis, Evgenia Tsoukala, Theodora D. Sarantopoulou, Maria‐Elpida Christopoulou   +3 more
wiley   +1 more source